掌叶半夏提取物的有效部位对体外培养宫颈癌细胞的促凋亡作用  

Apoptosis induction effect of Pinellia extract fraction on cell lines of cervical cancer cultured in vitro

作　　者：李桂玲[1] 归绥琪[1] 陈松华[2] 葛锡锐[2] 林理根[3] 叶阳[3] 

机构地区：[1]复旦大学附属妇产科医院,上海200011 [2]中科院上海生命科学院细胞资源中心,上海200031 [3]中科院上海药物研究所天然药化研究室,上海201203

出　　处：《中华中医药杂志》2008年第5期447-449,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市科委医学引导项目基金资助(No.074119613)

摘　　要：目的:观察中药掌叶半夏提取物的有效部位(pinellia extract fraction,PE)对体外培养的两种宫颈癌细胞株的作用,力求寻找一种能有效防治女性宫颈病变的纯中药制剂。方法:将4种不同浓度PE分别作用于Hela和Caski细胞1-5天,用倒置相差显微镜观察细胞生长及摄影,溴化噻唑蓝(MTT)比色法检测细胞活性,流式细胞术检测细胞凋亡。结果:PE对两种宫颈癌细胞株的生长均表现出明显的抑制作用,流式细胞术检测结果显示PE作用后两种细胞株均呈现出凋亡,且随着作用时间延长,凋亡细胞的比例明显增加。结论:PE对两种宫颈癌细胞株有明显的抑制生长和促凋亡作用;为进一步研究PE的药物作用机制和新药的研制开发奠定了初步的基础。Objective： To investigate the apoptosis induction effect of Pinellia extract fraction（PE） on two cell lines of cervical cancer cultured in vitro, in order to find a pure traditional Chinese reagent to treat cervical cancer effectively. Methods： PE was incubated in different concentrations with cervical cancer cell lines HeLa and CaSki. We tested the effects of PE on cell proliferation by MTT assay. The effects of PE on morphology, and cell cycle were studied by phase-contrast microscope and flowcytometry （FCM）. Results： PE could obviously inhibit the proliferation of HeLa and CaSki cells in a time and dose dependent manner. It could induce apoptosis of HeLa and CaSki. Conclusions： PE can suppress proliferation of cervical cancer cells and induce apoptosis. It may be a pure traditional Chinese reagent with non-side effects to prevent and treat cervical cancer effectively.

关 键 词：掌叶半夏 提取物 凋亡 宫颈癌 HeLa细胞株和CaSki细胞株 

分 类 号：R285[医药卫生—中药学] R737.33[医药卫生—中医学]