好氧颗粒污泥藻酸盐提取物的聚集形态研究  

Self-aggregation Property of Bacterial Alginates Extracted from Aerobic Granules

作　　者：林跃梅[1] 王琳[1] 

机构地区：[1]中国海洋大学环境科学与工程学院,青岛266100

出　　处：《环境科学》2008年第5期1181-1186,共6页Environmental Science

基　　金：国家自然科学基金项目(30570339);教育部科学技术研究重点项目(106105)

摘　　要：为探寻细菌藻酸盐对好氧污泥颗粒化的作用,借鉴海藻酸盐的提取方法,将好氧颗粒污泥中的细菌藻酸盐提取和鉴定后,对10-500 mg·L^-1范围内不同浓度的藻酸盐提取物在50 mg·L^-1 CaCl2溶液中的聚集形态进行了原子力显微镜观察.结果表明,细菌藻酸盐占好氧颗粒污泥干重的35.1%±1.9%.在CaCl2作用下,藻酸盐提取物具有从无序聚集成有序状态的超分子自组装特性,随着浓度的增加,藻酸盐提取物自发聚集,由纳米级的胶束微球聚集为微米级的花状,进而连接成网状成层堆积.好氧颗粒污泥中细菌藻酸盐通过形成具有三维空间结构的细菌藻酸盐-金属凝胶而对颗粒污泥的形成起骨架作用.其中,细菌藻酸盐-Ca^2＋凝胶在颗粒污泥的形成和维持其稳定结构中起主要作用.To explore bacterial alginates role in aerobic granules＇ formation, the alginate was extracted from aerobic granules and identified. Its aggregation property in 50 mg·L^- 1 CaCl2 solution was investigated by the atomic force microscopy （ AFM ）. Bacterial alginates amounted to 35.1% - 1.9% of granules＇ dry mass. With the concentration increased from 10 mg·L^- 1 to 500 mg·L^- 1 in 50 mg·L^- 1CaCl2 , the extracted alginates tended to form ordered aggregations, with the shape changed from randomly distributed globules, to rod-like and flower-shaped aggregations, and finally to weblike networks due to their supramolecular self-assembly property. The three dimensional alginate-metal gel is the structural polymer of aerobic granules, and the alginates-Ca^2＋ gel formation plays an important role in granules＇ formation and structure stabilization.

关 键 词：好氧颗粒污泥 细菌藻酸盐 超分子自组装 原子力显微镜 

分 类 号：X703.1[环境科学与工程—环境工程]