酮缬氨酸钙与酮异亮氨酸钙对人肾系膜细胞增殖影响及机制研究  

Effects of keto-valine-calcium and keto-isoleucine- calcium on proliferation of human mesangial cells and its mechanism study

作　　者：李洁 周平 胡秀梅 刘伏友[2] 彭佑铭[2] 

机构地区：[1]湖南省株洲市第一医院肾病内科,湖南株洲412000 [2]中南大学湘雅二医院肾病内科

出　　处：《中国医师杂志》2008年第4期489-491,共3页Journal of Chinese Physician

摘　　要：目的体外观察酮缬氨酸钙和酮异亮氨酸钙对人肾系膜细胞（HMC）的影响。方法10mM酮缬氨酸钙和酮异亮氨酸钙对HMC体外干预，检测干预后转化生长因子-β1（TGF-B1）和血管紧张素Ⅱ-1型受体（AT1R）的变化，并采用MTT和流式细胞仪检测细胞增殖、凋亡和细胞周期。结果10mM酮缬氨酸钙和酮异亮氨酸钙干预HMC，实验组与对照组相比，TGF-β1含量增加；细胞AT1R表达亦有增加；同时细胞出现增殖抑制，细胞流式分析发现，实验组干预后出现了细胞周期G1期阻滞并观测到了细胞凋亡。结论10mM的酮缬氨酸钙和酮异亮氨酸钙可以增加AT1R在HMC的表达，诱导TGF-β1的表达增加；同时诱导细胞周期G1阻滞并伴随细胞凋亡。Objective To study the effects of keto-valine-calcium and keto-isoleucinecalcium on （human mesangial cells） HMCs. Method HMCs were stimulated by keto-valine-calcium and keto-isoleucine-calcium. The AT1R and TGF-β1 were detected. MTT method was used to measure the proliferation of HMCs, and cell cycle was studied by flow cytometry. Results The expression of AT1R and TGF-β1 was increased in the experiment groups compared with negative and DMSO control groups. Cell cycle G1 arrest and cell apeptosis were observed in the experiment groups. Conclusions lOmM keto-valine-calcium and keto-isoleucine-calcium have multiple effects on HMCs in vitro, which not only increased the expression of AT1R, but also the expression of TGF-β1. Furthermore, keto-valine-calcium and keto-isoleucine-calcium can induce cell cycle G1 arrest and cell apoptosis.

关 键 词：缬氨酸 亮氨酸 肾小球膜 细胞增殖 

分 类 号：R96[医药卫生—药理学]