三聚β肽聚乙二醇化后抗肿瘤转移生物活性研究  

Effect of the Pegylation of Trimeric β Peptide on It's Anti-metastasis Activity

作　　者：朱珺[1] 王松梅[2] 刘银坤[3] 

机构地区：[1]上海交通大学附属胸科医院药剂科,上海市200030 [2]复旦大学上海医学院分子生物实验室,上海市200032 [3]复旦大学附属中山医院肝癌研究所,上海市200032

出　　处：《中国药房》2008年第13期970-972,共3页China Pharmacy

基　　金：国家863计划(2001A215411,2004AA215201);上海市现代生物与新药产业发展基金资助项目(024319212)

摘　　要：目的:比较聚乙二醇(PEG)修饰对三聚β肽(β3)抗肿瘤转移活性的影响。方法:采用黏附试验观察β3及其PEG修饰物(β3-PEG)对肝癌细胞株与纤连蛋白(FN)黏附能力的影响;采用人工基底膜胶观察β3和β3-PEG对肿瘤细胞侵袭重组基底膜能力的影响。结果:β3和β3-PEG对肿瘤细胞SMMC-7721和HCCLM6与FN黏附能力均有显著的抑制作用(P<0.05),且呈剂量及时间正相关;PEG修饰可增强β3对2种肿瘤细胞的黏附抑制作用(P<0.05)。β3和β3-PEG对2种肿瘤细胞迁移和侵袭均有显著的抑制作用(P<0.05)。结论:PEG修饰可增强β3抗黏附作用,但对细胞迁移和侵袭的抑制作用无明显影响。OBJECTIVE： To observe the effect of the polyglycol （PEG） modification of trimeric β peptide（β3） on it＇s anti - metastasis ability. METHODS： Using adhesion test to study the effects of β3 and β3- PEG on the adhesion of tumor cells to FN. Using artificial basal membrane to study the effects of β3 and β3- PEG on the invasion and recombination of basal membrane of tumor cells. RESULTS： Comparing to negative control, β3 and β3-PEG could both inhibit the adhesion of SMMC- 7721 and HCCLM6 tumor cells to FN with time- dependently（P〈 0.05） . The inhibitory effect of pegylated β3 peptide was stronger than that of β3 peptide（P 〈 0.05） . The mobility and invasion of HCCLM6 and SMMC- 7721 tumor cells were inhibited obviously by β3 and β3-PEG（P 〈 0.05）. CONCLUSION： PEG modification could enhance the anti -adhesion effect of β3, but its inhibitory effect on the mobility and invasion of two kinds of tumor cells was not remarkable.

关 键 词：三聚β肽 聚乙二醇化 黏附分子 肿瘤细胞 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]