FSGS大鼠骨髓内皮祖细胞数量和功能的变化及其意义  

Changes in number and function of bone marrow-derived EPCs focal segmental glomerulosclerosis rats

作　　者：赵洪雯[1] 余荣杰[1] 刘宏[1] 彭侃夫[1] 吴雄飞[1] 

机构地区：[1]第三军医大学西南医院肾科,重庆400038

出　　处：《重庆医学》2008年第8期805-807,898,899,共5页Chongqing medicine

摘　　要：目的观察阿霉素肾病大鼠骨髓内皮祖细胞(endothelial progenitor cells,EPCs)数量和功能的改变,探讨EPCs在局灶节段性肾小球硬化(FSGS)发生发展中的可能意义。方法选择阿霉素诱导的FSGS大鼠(FSGS组)和正常对照组大鼠(对照组)各10只,密度梯度离心法获得骨髓单个核细胞,将其接种在培养板培养6d,对贴壁细胞进行免疫细胞化学分析。激光共聚焦显微镜鉴定FITC标记的荆豆凝血素1(FITC-UEA-1)和DiI标记的乙酰低密度脂蛋白(DiI-acLDL)免疫双荧光阳性细胞为正在分化的EPCs。改良的Boyden小室和黏附能力测定观察EPCs的迁移和黏附能力。结果阿霉素诱导的FSGS大鼠骨髓EPCs数量明显减少〔EPCs/(视野×400)分别为(30.2±2.4)与(51.1±13.9),P<0.05〕,且FSGS组大鼠骨髓EPCs的黏附和迁移能力明显受损。结论FSGS的发生、发展可能与骨髓EPCs数量减少、迁移、黏附功能减退有关。Objective To investigate the changes on number and function of bone marrow-derived endothelial progenitor cells （EPCs） in Adriamycin induced focal segmental glomerulosclerosis （FSGS） rats,and study the potential role of EPCs in the development of FSGS. Methods Twenty SD rats were divided randomly into FSGS group （n = 10） and normal control group （n = 10）. Mononuclear cells were harvested from bone marrow and seeded on the culture plates. Immunocytochemical analysis was conducted after 6 days＇ culture. EPCs were characterized as DiI-acLDL and FITC-UEA-1 double positive cell detected by Laser Confocal microscopy. The migration function of EPCs was determined by Boyden chamber. The adherent function of EPCs was determined by counting the number of recultured EPCs, Results The number of EPCs was significantly reduced in the FSGS group rats, （30.2± 2.4） vs （51.1±13.9）,P〈 0.05. The functional activities of EPCs such as migration and adhesion were also impaired in FSGS rats. Conclusion The reduction of the number and the dysfunction of migration and adhesion of EPCs may be correlated with the pathogenesis and development of FSGS.

关 键 词：阿霉素肾病 骨髓 内皮祖细胞 

分 类 号：R692.6[医药卫生—泌尿科学]