RNA干扰抑制缺氧状态下单核细胞低氧诱导因子的表达  

RNAi silence on HIF-1 expression of alternative active mononuclear cell in hypoxgen condition

作　　者：何以平[1] 龚畅[2] 唐小江[1] 宋向荣[1] 蔡婷峰[1] 郑巧玲[1] 宋尔卫[2] 苏逢锡[2] 

机构地区：[1]广东省职业病防治院,广东广州510300 [2]中山大学附属第二医院乳腺外科,510120

出　　处：《中国职业医学》2008年第2期101-104,共4页China Occupational Medicine

基　　金：广东省医学科研基金课题(A2005059);高等学校博士学科点专项科研基金(新教师基金,20070558248)

摘　　要：目的将低氧诱导因子(HIF-1)的小干扰RNA(siRNA)导入从成人外周血中分离培养的单核细胞,观察其对缺氧状态下选择性激活的单核细胞HIF-1表达的影响。方法利用密度梯度离心的方法,从正常成人外周血中分离培养单核细胞并在低氧条件下充分激活,合成HIF-1-siRNA及多聚鸟苷酸(PTG)连结的siRNA(PTG-HIF-1-siRNA),体外转染充分激活的单核巨噬细胞,NorthernBlot、实时逆转录-聚合酶链反应检测目标基因的表达。结果PTG-HIF-1-siRNA能靶向导入低氧状态下选择性激活的巨噬细胞内;HIF-1-siRNA成功地抑制HIF-1的表达。结论RNA干扰抑制缺氧状态下的巨噬细胞HIF-1的表达,为进一步抑制巨噬细胞骨髓过氧化物酶(MPO)的表达,阻断苯的毒性代谢产物的生成提供了研究基础,为临床治疗苯中毒提供了新思路。Objective To observe the effect of HIF-siRNA on the HIF-1 expression of alternative activate blood mononuclear cell in hypoxygen condition. Methods Peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation, and activated in hypoxgen condition, then transfected with HIF-1-siRNA and PTC-HIF-1-siRNA. mRNA of HIF-1 were determinated by Northern Blot and RT-PCR. Results PTG-HIF-1-siRNA could be deliveried into AAMФs, and the expressions of HIF-1 were inhabited. Conclusion RNAi silences HIF-1 expressed by alternatively activated macrophages in hypoxgen.

关 键 词：RNA干扰 低氧诱导因子 单核细胞 

分 类 号：R994.3[医药卫生—毒理学] R135.1[医药卫生—药学]