广西来宾市五里塘普通野生稻(Oryza rufipogon Griff．)居群遗传多样性与核心种质研究  

Study on genetic diversity and nucleus germplasm of Wulitang population of common wild rice(Oryza rufipogon Griff.) in Laibin city of Guangxi

作　　者：黄金艳[1] 陈淼[1] 梁燕理[1] 刘驰[2] 陈英之[1] 马增凤[1] 黄大辉[2] 杨新庆[2] 李丹婷[2] 莫良玉[3] 李容柏[1] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室 [2]广西农业科学院水稻研究所,广西南宁530007 [3]广西大学农学院

出　　处：《西南农业学报》2008年第2期245-249,共5页Southwest China Journal of Agricultural Sciences

基　　金：广西科技创新能力与条件建设项目(桂科能05112001-1A1、0630006-5B、0630006-5F)

摘　　要：以位于北回归线穿越的广西中部西江流域的来宾市五里塘285份普通野生稻样本为材料,利用平均分布于水稻12条染色体上的24个微卫星标记进行遗传多样性分析。结果显示:24对微卫星标记引物共检测到73个等位基因,平均每对引物得到3个,平均多态信息含量(PIC)为0.4177,平均遗传杂合度(H)为0.4245,说明这些普通野生稻材料中有丰富的遗传多样性。通过逐步聚类分析,从285份材料中选出30份作为核心种质,该核心种质包含了24个微卫星标记能够检测到的所有基因突变位点,基本上可以代表该居群的遗传多样性。研究结果对居群的种质评估、遗传多样性保护和资源利用提供了参考依据。鉴于广西来宾市五里塘普通野生稻遗传多样性较丰富,建议对该原生地的现有居群进行重点保护和利用。Twenty-four SSR loci which were distributed evenly on the 12 chromosomes of rice were selected to assess the genetic diversity a- mong 285 accessions of common wild rice from Wulitang, Laibin city in the Xijiang valIey in central part of Guangxi Zhuang Autonomous Region, China, where the Tropic of Cancer is across over. A total of 73 alleles, ie. ,3 alleles per primer pair on average ,were detected in the population. The average polymorphism information content （PIC） and average heterozygosity（H） were 0. 4177 and 0. 4245 ,respectively. The resuits indicated that there existed abundant genetic diversity among these 285 accessions of Wulitang wild rice population. By stepwise duster analysis,30 accessions were selected out from the population as nucleus germplasm. This nucleus germplasm contained all the allelic variation in the population detected by the 24 SSR loci, and so could be representative for the genetic diversity of the population. It is suggested that the priority should be given to conservation in-situ and further utilization of the Wulitang common wild rice from Laibin city, especially the 30 accessions of the nucleus germplasm.

关 键 词：普通野生稻 核心种质 微卫星标记 遗传多样性 

分 类 号：S511.9[农业科学—作物学]