NE(MHS)纳米乳剂疫苗的抗肿瘤免疫学效应研究  

Anti-tumor immune responses of nanoemulsion-encapsulated MHS vaccine

作　　者：葛伟[1] 孙玉静[1] 李源[2] 张珊红[2] 张秀敏[1] 黄杨[1] 胡沛臻[1] 隋延仿[1] 

机构地区：[1]第四军医大学西京医院病理科 [2]第四军医大学西京医院老年病科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2008年第5期457-460,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30271464,30700994);陕西省攻关计划项目资助(2007K09-09-16);国家高技术研究发展计划(863)资助项目(2007AA02Z470)

摘　　要：目的:制备包裹有MAGE1-Hsp70和SEA的复合抗原的纳米乳剂疫苗NE(MHS)(nanoemulsion-encapsulated MAGE1-Hsp70/SEA),评价其抗肿瘤免疫学特性。方法:采用磁力超声法制备纳米疫苗NE(MHS),评价其粒径、包裹率及稳定性;NE(MHS)免疫动物,IFN-γ ELISPOT和LDH杀伤实验检测疫苗激活机体特异性细胞免疫反应情况;肿瘤攻击实验检测纳米乳剂疫苗的抗肿瘤效应。结果:(1)成功制备出粒径为(20±5)nm的NE(MHS),其药物包裹率为87%,于4℃放置6个月后或3000r/min10min离心后无分层;(2)与其他组相比,NE(MHS)组小鼠脾淋巴细胞中分泌IFN-γ的T细胞数量明显增多(P<0.05),CTL对B16-MAGE-1的特异性杀伤作用明显增强;NE(MHS)组B16-MAGE-1肿瘤成瘤时间长、成瘤率低。结论:NE(MHS)纳米乳剂疫苗具有良好的理化性质,能够刺激机体产生强烈的MAGE-1特异性的细胞免疫,能有效预防表达MAGE-1的肿瘤细胞攻击,是一种很有希望的新型抗肿瘤疫苗。AIM： To prepare nanoemulsion-encapsula- ted MAGE1-Hsp70/SEA and to evaluate its anti-tumor effects in mouse. METHODS： Nanoemulsion vaccine NE （MHS） was prepared using magnetic ultrasound methods and used to immunize C57BL/6 mice. The cellular immune responses were detected by IFN-γ ELISPOT and LDH release assay. The tumor challenge assay was performed too. RESULTS： （1） The mean size of NE（MHS） was （20 ±5） nm. The encapsulation rate was 87% and the nanoemulsion vaccine had a good stability. （2） The frequency of MAGE-1 specific CTL and cytotoxicity of CTL to B16-MAGE-1 cells were both greatly enhanced in immunization group than those in control group （ P 〈 0.05）. NE （MHS） could significantly delay the appearance of tumors and increase the percentage of tumor-free mice. CONCLUSION： The nanoemulsion had excellent physical and chemical characteristics. It could elicit MAGE-1-specific cellular immune response and anti-tumor effects against the MAGE-1-expressing tumor.

关 键 词：纳米乳剂 肿瘤疫苗 MAGE1 HSP70 SEA 

分 类 号：R392.11[医药卫生—免疫学]