抗PTD-bcr/abl单克隆抗体的制备及鉴定  

Preparation and characterization of the monoclonal antibodies to PTD-bcr/abl

作　　者：陈任安[1] 刘利[1] 蒋姗姗[1] 梁英民[1] 

机构地区：[1]第四军医大学唐都医院血液科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2008年第5期488-490,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：第四军医大学基金资助项目(CX02A023)

摘　　要：目的:制备单克隆抗体(mAb)并初步用于PTD-bcr/abl融合蛋白的研究,为慢性粒细胞白血病的免疫治疗提供实验依据。方法:在大肠杆菌中表达PTD-bcr/abl融合蛋白,用所获得的蛋白免疫BALB/c小鼠,常规收集小鼠脾细胞与Sp2/0细胞融合,筛选杂交瘤细胞株的阳性克隆,并对其进行一系列鉴定。结果:(1)表达PTD-bcr/abl融合蛋白;(2)制备了抗PTD-bcr/abl融合蛋白mAb;(3)用mAb以多种方法检测PTD-bcr/abl融合蛋白,证实此抗体效价高,特异性强。结论:此抗体可用于PTD-bcr/abl融合蛋白的进一步研究。AIM： To Preparation and characterization the monoclonal antibody （mAb） to PTD-bcr/abl fusion protein. METHODS： The PTD-bcr/abl fusion protein was expressed in E. coli. BALB/c mice were immunized with specific PTD-bcr/abl. The splenic cells of the mice were fused with Sp2/0 mouse myeloma cells. The hybridoma culture supernatants were screened by indirect ELISA. The property of the antibody was identified by Western blot and immunohistochemistry. RESULTS： The PTD-bcr/abl fusion protein and its mAb were obtained, mAb against PTD-bcr/abl displayed strong specifllity and high affinity. CONCLUSION： An anti-PTD-bcr/abl fusion protein mAb with high affinity was abtained, which could be applied to the research of PTD-bcr/abl.

关 键 词：PTD-bcr/abl融合蛋白 单克隆抗体 制备 

分 类 号：R392.11[医药卫生—免疫学]