黄芪、党参提取物对小鼠Matrigel种植体血管生成的影响  

Effect of huangqi and dangshen extraction on angiogenesis in Matrigel implant of mice

作　　者：柏长青[1] 宋颖芳[2] 简文[2] 王德堂[2] 马存保[3] 

机构地区：[1]军事医学科学院附属医院呼吸内科,北京100071 [2]第四军医大学西京医院,陕西西安710032 [3]西北工业大学计算机学院,陕西西安710072

出　　处：《细胞与分子免疫学杂志》2008年第5期498-499,501,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：中国临床肿瘤学科学基金资助项目(Y-2005-0035)

摘　　要：目的:研究黄芪、党参提取物对血管生成的影响,为其抗肿瘤应用提供实验依据。方法:采用b-FGF诱导小鼠腹壁Matrigel种植体方法进行血管生成实验,Matrigel与b-FGF、肝素混合后植入C57BL/6小鼠腹壁正中区域皮下,5d后取出,分别测量Matrigel种植体中血红蛋白含量和微血管面积,以此反应微血管的生成状况。在Matrigel种植前3d,给予不同剂量的实验药物,连用8d进行抑制实验。结果:低剂量(≤50%,V/V)黄芪、党参提取物,能促进Matrigel种植体内的血管生成,而高剂量(≥60%,V/V)则表现出抑制现象。这种促进和抑制作用具有一定的剂量-效应关系。结论:不同剂量的黄芪、党参提取物对微血管的生成具有不同的影响,表现出中医药特有的双向调节作用。临床使用时应根据不同的目的,选择不同的药物剂量。AIM： To study the inhibitory effect of huangqi and dangshen extraction （SQ） on angiogenesis induced by b-FGF. METHODS： Matrigel implant assay was used. Matrigel（500 μL） containing b-FGF and heparin was injected subcutaneously into the abdomens of mice and harvested 5 d later. The amount of hemoglobin and micro-vascular area present in the implant were measured and compared. The mice were given defferent dosage of SQ （experimental group） or the same volume of glucose （vehicle group） once a day by intraperitoneal injection. Inhibitory experiment started 3 d before Matrigel implant and continued until the end of study. RESULTS： SQ in lower dosage （ ≤50% V/V） increased hemoglobin content and micro-vascular area in Matrigel implant while SQ in higher dosage （ ≥60%, V/V） reduced hemoglobin content and micro-vascular area in Matrigel implant. The effect of enhance ment and inhibition was in a limited concentration-effect manner. CONCLUSION： SQ in different dosage has differente effects on angiogenesis. We should use different dosage in different purpose.

关 键 词：黄芪 党参 血管生成 

分 类 号：R979.14[医药卫生—药品]