超支化聚合物涂层毛细管柱分离蛋白质的研究  

Hyperbranched polymers coated CE columns and their applications to separetion of protein

作　　者：宋南京[1] 张志良[2] 尚盼[1] 徐文超[1] 娄嵩[1] 寿崇琦[1] 

机构地区：[1]济南大学化学化工学院,山东济南250022 [2]山东轻工业学院分析检测中心,山东济南250353

出　　处：《山东轻工业学院学报（自然科学版）》2008年第1期65-68,共4页Journal of Shandong Polytechnic University

基　　金：山东省自然科学基金资助项目(G0645)

摘　　要：合成了以三羟甲基丙烷为核的脂肪族超支化聚醚,并对其进行了红外表征,羟值测定。通过物理吸附方法将其涂布在熔融石英毛细管内表面,测定了涂层柱的电渗流及其对碱性蛋白质的分离能力。结果表明,涂层柱能有效的抑制电渗流及蛋白质在毛细管内壁上的吸附;在pH=5.0的磷酸盐缓冲溶液中,16 kV的操作电压下,对三种碱性蛋白质实现了基线分离,其中G4超支化聚醚涂层柱的分离柱效达到了106plates/m。A series of aliphatic hyperbranched polyethers were synthesized by using the trimethylol propane as core molecule,and their molecular structures were characterized by IR, hydroxyl value. These polymers were coated on the inner surface of fused silica capillaries by physical adsorbtion, and their electroosmotic flow and separation capability for basic proteins were investigated. The results demonstrated the coated columns were good enough to repress the electroosmotic flow and the adsorption of the proteins on the capillary wall. Three proteins compound were separated successfully by using the buffer of pH 5.0 and pressure of 16 kV. The separation efficiencies of G4 coated column reached 106 plates/m.

关 键 词：超支化聚醚 毛细管电泳 蛋白质 涂层柱 分离 

分 类 号：TQ028[化学工程]