大鼠脊髓半横断损伤后硫酸软骨素蛋白多糖Versican的变化  

Changes in the expression of versican of rat with hemisection of spinal cord injury

作　　者：赵伟[1] 张连双[1] 章为[2] 彭谨[2] 

机构地区：[1]滨州医学院组织学与胚胎学教研室,山东烟台264003 [2]四川大学华西基础医学与法医学院组织胚胎与神经生物学教研室,四川成都610041

出　　处：《解剖学研究》2008年第2期105-107,111,共4页Anatomy Research

摘　　要：目的观察大鼠脊髓半横断损伤(hSCI)位点头尾段硫酸软骨素蛋白多糖Versican的表达变化,探讨其与脊髓损伤修复的关系。方法20只成年健康SD大鼠随机分为假手术对照组和脊髓半横断损伤3d、7d和14d组。建立成年SD大鼠脊髓半横断(T9～T10)模型。取损伤位点头尾段T9、T10节段制作冰冻切片,运用Versican抗血清进行免疫组化ABC法染色。分别观察并计数各组前角中Versican阳性细胞数,采用计算机图像分析技术测量免疫阳性产物的平均光密度(A)值。结果Versican阳性产物主要分布于细胞外基质中,前角神经元和胶质细胞的胞浆中也有分布;损伤后前角中Versican阳性神经元和胶质细胞数较对照组明显减少(P<0.05),免疫阳性产物平均A值较对照组明显增加(P<0.05)。结论脊髓半横断损伤后,表达增加的Versican很可能参与损伤后轴突生长被抑制的过程。Objective To investigate the expression changes of Versican of CSPGs family in spinal cord in 3d, 7d and 14d following hemisection of spinal cord injury （hSCI） and to explore the relationships of Versican and spinal cord injury. Therefore, to offer the morphological datum for the research concerning the spinal cord repair. Methods 20 adult healthy Sprague-Dawley rats were randomly divided into sham-operated control group and 3 d, 7 d, 14 d spinal cord injury groups. The spinal cords were hemisected between T9 and T10. The spinal cord was removed from the rostral and caudal segments of lesioned areas. Then the tissue blocks were made into 25μm frozen sections and the immunohistochemistry ABC method was performed on these sections. We respectively observe and count the number of Versican positive cells in ventral horn of spinal cord for all groups, and the average A value of immunoreactant were detected with computer image analysis technique. Results Versican positive products were mainly distributed in neurons and neuroglial ceils in spinal cord ventral horn for the control group, and were also distributed in extracellular matrix for Versican. The Versican positive cells of rostral or caudal part of injury site for the three spinal cord injury groups were all decreased in number （P〈0.05）. The average A values for all three spinal cord injury groups were increased （P〈0.05）. Conclusion The increased-expression Versican may mutually play inhibitory effect on axon regeneration following SCI.

关 键 词：脊髓半横断损伤 VERSICAN 硫酸软骨素蛋白多糖 大鼠 

分 类 号：R651.2[医药卫生—外科学]