HPLC法同时测定丹参安神胶囊中丹参素钠和原儿茶醛的含量  

Determination of sodium danshensu and protocatechuic aldehyde in Danshen Anshen Capsules by HPLC

作　　者：邱素民[1] 徐新民[2] 陈福民[3] 

机构地区：[1]金乡县人民医院,山东金乡272200 [2]兖州市人民医院,山东兖州272100 [3]嘉祥县人民医院,山东嘉祥272400

出　　处：《齐鲁药事》2008年第4期214-215,共2页qilu pharmaceutical affairs

摘　　要：目的建立用HPLC法同时测定丹参安神胶囊中丹参素钠和原儿茶醛含量的检测方法。方法研究丹参安神胶囊中丹参素钠和原儿茶醛的含量检测方法。ODS为固定项;甲醇-0.5%冰醋酸溶液(17?83)为流动相;检测波长280nm。结果丹参素钠在20～200μg.mL-1浓度范围内,浓度与峰面积呈良好的线性关系(r=0.9997),平均回收率为100.53%(RSD=0.796%);原儿茶醛在4～40μg.mL-1浓度范围内,浓度与峰面积呈良好的线性关系(r=0.9995),平均回收率为98.80%(RSD=1.287%)。结论本法简便、快速、准确、灵敏,重现性好,可以用于控制丹参安神胶囊的质量。OBJECTIVE To establish an HPLC method for determination of sodium danshensu and protocatechuic aldehyde in Danshen Anshen Capsules. METHODS Study on determination method of sodium danshensu and protocatechuic aldehyde in Danshen Anshen Capsules. Stationary phase was ODS;mobile phase was composed of methanol-0. 5 %glacial acetic acid solution（17 ： 83） ;UV detection wavelength was 280 nm. RESULTS The linear ranges of sodium danshensu and protocatechuic aldehyde were 20-200μg · mL^-1 and 4-40μg ·mL^-1, respectively;the correlation coefficients were 0. 9997 and 0. 9995, respectively; the average recoveries were 100.53% （ RSD = 0. 796%） and 98.80% （ RSD = 1. 287%）, respectively. CONCLUSION The method was simple, rapid, accurate, sensitive and reproducible. It was suitable for the determination of tanshinol sodium and protocatechualdehyde in Danshen Anshen Capsules.

关 键 词：丹参安神胶囊 丹参素钠 原儿茶醛 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]