红龙草组织培养及快繁技术  

Studies on Tissue Culture and Rapid Propagation of Altemanthera ficiode cv.'Ruliginosa'

作　　者：黄海泉[1] 陈晓华[1] 刘齐元[2] 黄美娟[1] 

机构地区：[1]西南林学院园林学院,云南昆明650224 [2]江西农业大学农学院,江西南昌330045

出　　处：《江西农业大学学报》2008年第2期235-238,256,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：云南省自然科学基金(2005C0004M);云南省高校园林植物与观赏园艺重点实验室项目

摘　　要：以红龙草(Altemanthera ficiodecv.‘Ruliginosa’)未木质化茎段为外植体建立无菌体系,并对其丛芽增殖及生根诱导进行了研究,结果表明:红龙草未木质化茎段较好的灭菌方法为:φ=75%酒精30 s+1 g/L升汞5.5 min;较好的诱导丛芽增殖的培养基为:MS+KT1.5 mg/L+NAA0.01 mg/L+蔗糖20 g/L;较好的诱导生根培养基为:1/2MS+NAA0.5 mg/L。The annual unlignified tender stems of Altemantheraficiode cv. ‘ Ruliginosa’ were used as explants and inoculated into the primary culture medium, which obtained its aseptic plantlets in vitro. Furthermore, its proliferation and rooting culture were studied further. The results indicated that the optimal sterilization method for unlignified tender stems of Altemanthera ficiode cv. ‘Ruliginosa’was 75%ethanol 30 s ＋ 1 g/L HgC1 5.5 min among all tested ones; that its optimal proliferation culture medium among all tested culture media was MS ＋ KT 1.5 mg/L ＋ NAA 0.01 mg/L ＋ sucrose 20 g/L; that its best rooting culture medium among all tested ones was 1/2MS ＋ NAA0.5mg/L.

关 键 词：红龙草 组织培养 快繁 

分 类 号：S543.9[农业科学—作物学] S604.3