鳄龟科和平胸龟科线粒体控制区序列分析和结构比较  

Comparison of Mitochondrial Control Region Sequences Between Chelydridae and Platysternidae

作　　者：颜亮[1] 张雁[1] 汪宁[1] 张莉[1] 聂刘旺[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽重要生物资源保护与利用研究重点实验室,安徽芜湖241000

出　　处：《Zoological Research》2008年第2期127-133,共7页动物学研究（英文）

基　　金：国家自然科学基金(30640048和30770296);安徽省科学与技术后备人选基金项目(2006-2);"生物环境与生态安全"安徽省高校重点实验室基金(2006)~~

摘　　要：本文参照龟类近缘种的线粒体DNA(mitochondrial DNA,mtDNA)控制区(control region,CR)及邻接序列,设计了二对特异引物,采用PCR和测序技术,获得了大鳄龟(Macroclemys temminckii)、小鳄龟(Chelydra serpentina)和平胸龟(Platysternon megacephalum)mtDNA CR区序列,其长度分别为1062bp、1124bp和1119bp;A+T的含量分别为68.93%、69.34%和69.44%。序列分析显示,三种龟CR区3'末端均存在丰富的微卫星序列,其中大鳄龟和小鳄龟各有一段2bp的TA序列分别重复20和15次;小鳄龟另有一段5bp的TATAT序列重复13次;平胸龟则是一段10bp的AGTATGTTAT序列重复4次和一段17bp的GTTGTTATATAACATAT序列重复13次。本文还结合GenBank中已发表的其他6种龟鳖类动物的控制区序列,探讨了龟鳖类动物微卫星序列的类型及分布,结果表明:9种龟鳖类动物都存在丰富的微卫星序列,且微卫星所在位置及序列存在很大差异。The sequences of mitochondrial control region （CR） of Macroclemys temminckii, Chelydra serpentina and Platysternon megacephalum were obtained using PCR and sequencing techniques, with gene-specific primers, based on the CR and its flanking sequences from other species. The CR lengths of the three species were 1089bp, 1124bp and 1119bp respectively, and the base composition of A＋T were 68.97%, 69.34% and 69.44% respectively. One fragment of （TA）20 microsatellite was found in M. temminckii; one fragment of （TATAT） 13 direct tandem repeats followed by （TA）15 microsatellites were found in C.serpentina; and one fragment of （AGTATGTTAT）4 direct tandem repeats followed by （GTTGTTATATAACATAT） 13 repeats were found in P. megacephalum. The distribution of mtDNA microsatellites in tortoises was discussed based on the CR sequences of the three species and other six tortoises published in GenBank. The result suggested that all the nine tortoises have microsatellites, and there are obvious differences both at the site and the sequences.

关 键 词：大鳄龟 小鳄龟 平胸龟 线粒体DNA 控制区 微卫星 

分 类 号：Q953[生物学—动物学]