长春花花叶病病原物的分离和鉴定  

Isolation and Identification of the Pathogen That Causes Mosaic on Periwinkle

作　　者：曹言勇[1] 施艳[1] 范在丰[1] 宋丽敏[2] 田国忠[2] 

机构地区：[1]中国农业大学植物病理系农业部分子病理学重点开放实验室,北京100094 [2]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091

出　　处：《林业科学》2008年第4期60-68,共9页Scientia Silvae Sinicae

基　　金：国家科技基础条件平台建设项目(2005DKA21207)

摘　　要：长春花病原物采自海南省琼海市博鳌镇和广东省广州市星海公园,症状表现为系统花叶与畸形,对其进行染病外植体组织培养、鉴别寄主反应、传播方式鉴定、病毒粒体形态观察和血清学反应及病叶双链RNA(dsRNA)分析。引起长春花花叶病的病原物鉴定为黄瓜花叶病毒(cucumber mosaic virus,CMV),克隆该病毒分离物外壳蛋白基因核苷酸序列,并对该核苷酸序列进行生物信息学分析,结果显示侵染我国长春花引起长春花花叶和畸形的CMV分离物应初步划归在CMVIB亚组。The systemic mosaic and malformation symptoms were observed on the leaves of periwinkle （ Catharanthus roseus） from Bo＇ ao, Qionghai, Hainan Province and Xinghai Park, Guangzhou, Guangdong Province. With the infected tissue culture, host range determination, serological assay and viral particle morphological observation, dsRNA analysis, the pathogen was identified to be cucumber mosaic virus （ CMV）. Cloning of the coat protein gene and analysis of the nucleotide sequences further demonstrated that this CMV isolate was tentatively assigned to the IB subgroup of CMV.

关 键 词：长春花 花叶病 黄瓜花叶病毒 

分 类 号：S763.11[农业科学—森林保护学]