冷暴露对中缅树鼩肝脏、膈肌和心肌线粒体呼吸的影响  

Effects of cold exposure on mitochondrial respiration of liver,diaphragm and cardiac muscle in tree shrews (Tupaia belangeri)

作　　者：谢静[1] 王颖[1] 练销[1] 王政昆[1] 

机构地区：[1]云南师范大学生命科学学院,生物能源持续开发利用教育部工程研究中心,昆明650092

出　　处：《兽类学报》2008年第2期157-164,共8页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(30260021,30560026);云南省重点应用项目

摘　　要：在4℃急性冷暴露(1h,4h,8h,24h)和持续冷暴露(7d,14d,28d)条件下,测定中缅树鼩膈肌、心肌和肝脏的线粒体状态Ⅲ呼吸、状态Ⅳ呼吸、呼吸控制率(RCR)、线粒体蛋白含量以及肝脏线粒体P/O值的变化。结果表明:肝脏线粒体状态Ⅲ、状态Ⅳ呼吸随着低温处理时间的延长,呼吸速率均极显著增加,在28d后分别增加了132.9%、124.4%(P<0.01),RCR与对照比较,在8h和7d组分别显著增加了35.8%和48.4%(P<0.05),线粒体蛋白含量也极显著增加,在28d后增加了104.7%(P<0.01),P/O值在整个低温处理过程中呈下降趋势,在28d后降低了40.2%,达到极显著水平(P<0.01);膈肌线粒体状态Ⅲ呼吸在整个低温处理期间没有显著变化,状态Ⅳ呼吸在28d达到极显著增加(P<0.01,64.9%),RCR在28d后显著降低(P<0.05,42.1%),线粒体蛋白只有4h组有极显著增加(P<0.01,45.2%);心肌的状态Ⅲ呼吸在8h组有着极显著的增加(P<0.01,54.7%),状态Ⅳ呼吸随着低温处理时间的增加而显著增加,28d后增加了94.7%(P<0.01),RCR在28d后降低37.8%(P<0.01),线粒体蛋白表现出先下降再上升的趋势,8h组下降37.8%(P<0.01),28d增加25.2%(P<0.05)。说明中缅树鼩在冷胁迫的条件下肝脏线粒体呼吸能力显著增强,主要表现为状态Ⅳ呼吸即质子漏产热的显著增强,膈肌和心肌的线粒体呼吸也具有一定的适应性变化,补偿了冷胁迫条件下中缅树鼩增加的能量需求,是中缅树鼩在冷胁迫中重要的适应对策。The state Ⅲ respiratory ability, state Ⅳ respiratory ability, respiration control ratio （RCR） , total protein content and mitochondrial protein contents of liver, diaphragm and cardiac muscle in tree shrews （ Tupaia belangeri） were measured during acutecy cold exposure for 1 h, 4 hs, 8 hs, 24 hs, and prolonged cold exposure for7 days, 21 days and 28 days. The state Ⅲ respiratory ability, state Ⅳ respiratory ability were measured by oxygen electrodes and the total protein content and mitochondrial protein contents were measured by Lowry method. The liver mitochondrial state Ⅲ and Ⅳ respiratory ability both greatly increased with prolonged cold exposure, 132.9% and 124.4% （ P 〈 0. 01 ） respectively increased after 28 d. Compared to the controls, RCR increased by 35.8% and 48.4% （P 〈 0. 05） in the 8 h and 7 d groups and the content of mitochondrial protein also increased by 104. 66% （P 〈0. 01 ） while the P/O value showed a decreasing trend during the whole cold exposure process, showing a very significant level after 28 d, decreasing by 40.2% （ P 〈 0. 01 ）. The respiration of the diaphragm muscle mitochondrial state Ⅲ did not change evidently during the cold exposure process, however, the state Ⅳ respiration increased by 54. 7% （P 〈0. 01 ） while RCR decreased by 42. 1% （P 〈 0.05 ） and the mitochondrial protein increased by 45.2% （ P 〈0. 01）. only in the 4 h group. The state Ⅲ respiration of the cardiac muscle mitochondria increased by 54. 7% （P 〈0. 01 ） in the8 h group and the state Ⅳ respiration significantly increased 94. 7% by the cold exposure （P 〈0.01 ） , while RCR decreased by 37.8% （P 〈0. 01 ） and the content of mitochondrial protein showed a trend that increased at first and decreased later and the 8 h group decreased by 37.8% （P 〈0. 01 ）, and increased by 25.2% （P 〈 0. 05 ） after 28 d. The mitochondrial respiration of liver was significantly enhanced during the cold stress incorporating that the increase of p

关 键 词：低温 中缅树鼩 膈肌 心肌 肝脏 线粒体呼吸 质子漏 

分 类 号：Q494[生物学—生理学]