性伴间淋球菌的opa分型及Ng—MAST基因分型  

Comparison of opa typing with Neisseria gonorrhoeae multi-antigen sequence typing for discrimination of Neisseria gonorrhoeae from patients and their sex partners

作　　者：陈宏翔[1] 吴志洪[1] 李家文[1] 刘厚君[1] 陈嵘祎[1] 严小枫[1] 帅俊[1] 许莉[1] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤性病科,武汉430022

出　　处：《中华皮肤科杂志》2008年第5期307-310,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30700717,30371293）

摘　　要：目的评价淋球菌opa分型及Ng—MAST对淋球菌分离株进行基因分型的能力，分析性伴分离淋球菌基因型别之间的关系。方法本研究中24株淋球菌野生株于2006年2月至2007年8月从华中科技大学同济医学院附属协和医院皮肤科门诊分离获得，经Thayer Martin（T—M）培养基选择分离培养后通过革兰染色和氧化酶试验等方法确证。PCR扩增其opa基因以HpaⅡ酶切及聚丙烯酰胺凝胶电泳银染显色分析，并用NG—MAST方法从分子水平上进行基因分型。结果24株野生株淋球菌中，Ng—MAST区分出10种ST型，而使用opa分型则呈现12种OT型；新发现的ST（217—86％同源178）是国内特有的流行株。结论淋病患者一性伴组除了45／46之外，每对菌株的ST型及OT型均相同，表明患者与性伴相互接触传染。淋球菌opa分型能力高于Ng—MAST，ST型别可进一步的分成opa—types（OT）亚型。Objective To compare the potentiality of opa typing versus Neissena gonorrhoeae multi- antigen sequence typing （Ng-MAST） in discrimination of N. gonorrhoeae isolates, and to investigate the consistency of genotypes of N. gonorrhoeae between patients and their sex partners. Methods N. gonorrhoeae was isolated from patients and their sex partners who attended sexually transmitted disease clinics of the Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology from February 2006 to August 2007. All isolates were cultured on Thayer-Martin agar and GC-based chocolate agar, and identified by Gram stain and oxidase reaction. Totally, twenty-four strains of N. gonorrhoeae were isolated form 12 pairs of male-female sex partners. Genomic DNA was isolated from the strains, and subjected to PCR amplification of opa gene as well as por and tbpB genes, opa typing and Ng-MAST typing were utilized to differentiate the 24 strains respectively. Results The 24 isolates were classified into 10 sequence types （ST） by Ng-MAST typing, and 12 opa types （OT） by opa typing. A novel genotype identified by Ng-MAST （217-86% homologisation 178）, was unique in China. Conclusions Both ST and OT of N. gonorrhoeae strains are identical between patients and their corresponding partners except for one pair of partners, 45/46, suggesting that gonorrhea is transmitted to each other by contiguity between sex partners. Opa typing is likely to be more potential than Ng-MAST in genotyping of N.gonorrhoeae strains.

关 键 词：奈瑟球菌 淋病 基因型 NG—MAST 治疗方法 

分 类 号：R450[医药卫生—治疗学] R759.2[医药卫生—临床医学]