水稻抗病基因介导的抗白叶枯病反应中蛋白质表达谱的比较分析  

Analysis of Protein Expression Profiling in Rice Disease Resistance Gene-Mediated Resistance to Xanthomonas oryzae pv.oryzae

作　　者：曾亚[1] 丁新华[1] 沈祥陵[1] 李香花[1] 王石平[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室/国家植物基因研究中心(武汉),湖北武汉430070

出　　处：《中国水稻科学》2008年第3期234-242,共9页Chinese Journal of Rice Science

基　　金：农业部948计划资助项目

摘　　要：采用双向电泳-质谱分析技术,比较分析了水稻在接种白叶枯病菌(Xanthomonas oryzae pv.oryzae)1、4、8、24、72h后蛋白质表达谱的差异。通过比较接种白叶枯病菌和对照之间、接种不同白叶枯病菌生理小种之间的表达差异,鉴定了72个差异表达的抗病相关蛋白点,对其中部分点的蛋白质进行了电离子喷雾二级质谱学分析鉴定,确定了11个抗病反应中差异表达的蛋白质。The protein expression profile during rice-Xanthornonas oryzae pv. oryzae （Xoo） interaction by using a two-dimensional electrophoresis-mass spectrometry strategy was analyzed. Proteins were extracted from rice leaves at 1, 4, 8, 48, and 72 hours after Xoo inoculation. Seventy-two differentially expressed protein spots were identified by comparing the expression profiles of proteins from Xoo- and mock-inoculated plants revealed by two-dimensional electrophoresis. Further analysis using electrospray ionization-time of flight mass spectrometry revealed the property of 11 of the 72 differentially expressed proteins. These results provide basis for further studying the molecular mechanism of rice disease resistance.

关 键 词：水稻 抗病性 蛋白质组学 白叶枯病菌 表达谱 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]