一个水稻钙调素结合蛋白基因OsCaMBP的克隆及表达分析  

Cloning and Expression Analysis of a Calmodulin-Binding Protein Gene from Rice

作　　者：万佳[1] 况浩池[2] 谢必武[3] 陈静 张琦[1] 徐永菊[1] 高晓玲[1] 徐正君[1] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]四川省农业科学院水稻高粱研究所,四川泸州646100 [3]重庆三峡职业学院生物工程系,重庆万州404001 [4]四川绵阳金地高科种业有限公司,四川绵阳621000

出　　处：《中国水稻科学》2008年第3期243-248,共6页Chinese Journal of Rice Science

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT0453);国家科技支撑计划资助项目(2007BAD81B00)

摘　　要：通过荧光差异显示和RT-PCR技术，克隆到一个新的水稻钙调素结合蛋白基因OsCaMBP。序列分析表明，该基因cDNA序列全长2094bp，编码一个具有569个氨基酸残基的多肽，推测分子量为63．2kD；在OsCaMBP蛋白的N端存在IQ钙调素结合构象，与其他植物中的钙调素结合蛋白的相似性介于38．25％-47．28％。在热激处理15min、30min、1h或2h后，该基因在水稻的叶、根和叶鞘中表达量急剧增加；此外，该基因表达量也受低温调控而增加。By using fluorescence differential display and RT-PCR methods, a new member of the calmodulin-binding proteins family of rice was isolated and termed as OsCaMBP. The full length cDNA was 2094 hp and encoded a polypeptide containing 569 amino acid residues （63.2 kD）. Amino acid sequence of the deduced protein revealed the existence of IQ calmodulin binding motif at the N-terminus. Similarity analysis indicated that rice OsCaMBP has 38.25 %-47.28 % identities with CaMBPs in other plants. Expression of the OsCaMBP gene was greatly increased in leaves, roots and leaf sheaths after the heat shock treatment at 42℃ for 15 min, 30 min, 1 h and 2 h. Furthermore, the gene expression accumulation was enhanced in response to chilling stress.

关 键 词：水稻 钙调素结合蛋白 荧光差异显示 基因克隆 表达 

分 类 号：S511[农业科学—作物学]