鸡MC4R基因新突变位点的发现  

Discovery of New Mutation Points in Chicken MC4R Gene

作　　者：陶勇[1] 王金玉[1] 李国辉[1] 胡玉萍[1] 

机构地区：[1]扬州大学动物科学与技术学院

出　　处：《安徽农业科学》2008年第11期4528-4529,4531,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省高新技术项目(BG2004316);江苏省"青蓝工程"培养基金资助项目

摘　　要：[目的]为深入研究鸡MC4R基因和分子育种提供参考。[方法]采用饱和酚/氯仿法从鸡血中提取DNA,利用PCR-SSCP和DNA测序的方法,对京海黄鸡MC4R基因的单核苷酸多态性进行分析。[结果]对PCR产物进行SSCP分析,2对引物均表现出多态性。引物A得到2种基因型(AA型和AB型)。引物Z也得到2种基因型(CC型和CD型)。将测序结果与发表的鸡MC4R基因序列的比较结果进行比较,发现2个单核苷酸多态位点,其中A引物扩增序列中的多态位点为662 bp处G→C的点突变造成的,而Z引物扩增序列中的多态位点是由于在733～734 bp插入了一个C碱基引起的。[结论]该研究中未检测到BB和DD纯合基因型,这可能与在京海黄鸡培育过程中采用分子标记方法进行选择有关。[Objective] The research aimed to provide reference for further study on chicken MC4R gene and its molecular breeding. [Method] DNA was extracted from chicken blood by using saturated hydroxybenzene/chloroform method. And the single nucleotide polymorphism of MC4R gene in Jinghai yellow chicken was analyzed by using the methods of PCR-SSCP and DNA sequencing. [Result] The PCR products were analyzed by SSCP and 2 pairs of primers showed the polymorphism. Two kinds of genotypes （AA type and AB type） were obtained frmn primer A and 2 kinds of genotypes （CC type and CD type） were obtained from primer Z. The comparison results between the sequencing results and the reported MC4R gene sequences showed that 2 single nueleotide polymorphic loci were found. The polymorphic loci in the amplified sequences of primer A was caused by point mutation from G to C at 662 bp and that in the amplified sequences of primer was caused by inserting one C base at 733-734 bp. [Conclusion] In this study, pure genotypes BB and DD were not detected, which was probably related with the molecular marker method used in the breeding of Jinghai yellow chicken.

关 键 词：京海黄鸡 MC4R基因 SNPS 

分 类 号：S831[农业科学—畜牧学]