rhG-CSF动员的健康供者骨髓采集当天外周血中单核细胞数量和骨髓采集物中CD34^+细胞数量关系的研究  

Correlation of monocyte counts prior to the collection of recombinant human granulocyte colony-stimulating factor primed bone marrow grafts with the quantities of CD34^+ cells in the grafts.

作　　者：常英军[1] 霍明瑞[1] 赵翔宇[1] 罗小华[1] 黄晓军[1] 

机构地区：[1]北京大学人民医院北京大学血液病研究所,北京100044

出　　处：《中国实用内科杂志》2008年第5期347-348,共2页Chinese Journal of Practical Internal Medicine

基　　金：国家"863"计划资助(2006AA02Z4A0)

摘　　要：目的探讨人重组粒细胞集落刺激因子(rhG-CSF)动员的健康供者骨髓采集当天外周血循环中单核细胞数量和骨髓采集物中CD34+细胞数量的关系。方法以2004年6月至2007年7月在北京大学人民医院接受异基因造血干细胞移植的53名亲缘健康供者为研究对象,所有供者均皮下注射rhG-CSF5μg/(kg·d),连续5d,第4天和第5天分别采集骨髓和外周血。骨髓采集前用EX2100血细胞分析仪检测外周循环血常规,流式细胞仪测定骨髓采集物中的CD34+细胞数量。结果rhG-CSF动员的53名供者骨髓采集当天的每微升外周血单核细胞的数量为(896±424)个。每微升骨髓采集物中的CD34+细胞是(84±45)个,CD34+细胞总量为(8.22±4.84)×107。Pearson相关分析显示,每微升外周血循环中的单核细胞数量与每微升骨髓采集物中CD34+细胞的量(r=0.573,P<0.01)、CD34+细胞总量(r=0.586,P<0.01)呈正相关关系;逐步线性回归模型分析显示,外周血循环中的单核细胞数量与每微升骨髓采集物中CD34+细胞的量(β=0.568,P<0.01)及CD34+细胞的总量(β=0.462,P<0.01)呈正相关关系。结论rhG-CSF动员的健康供者骨髓采集当天外周血循环中单核细胞数量是一项预测骨髓采集物中CD34+细胞数量的简易、可行的指标。Objective To investigate the correlation of monocyte counts prior to the collection of recombinant human granulocyte colony-stimulatlng factor（rhG-CSF） primed bone marrow grafts （G-BM）with the quantities of CD34^＋ cells in the grafts. Methods From June 2004 to July 2007, fifty-three healthy donors were treated with rhG-CSF [ 5 μg/（ kg · d） ] injected subcutaneously for five consecutive days. Bone marrow grafts and peripheral blood grafts were harvested on the 4th and 5th day,respectively. The quantities of CD34^＋ cells and blood routine prior to the collection of the G-BM were determined by flow cytometry and blood analyzer XL2100, respectively. Results The monoeyte counts in peripheral blood （PB） of the 53 healthy donors were 896 ± 424 per microliter. The counts of CD34^＋ cells per microliter and quantities of total CD34^＋ cells in the bone marrow grafts were 84 ± 45 and（8.22 ± 4. 84） × 10^7 ,respectively. Pearson correlation analysis indicated that the counts of monocyte in the PB correlated positively with the counts of CD34^＋ cells per microliter （r = 0. 573, P 〈 0. 01 ）and quantities of total CD34^＋ cells（ r = 0. 586, P 〈 0. 01 ）in the bone marrow grafts. A stepwise linear regression model also showed that the counts of monocyte in the PB correlated positively with the counts of CD34^＋ cells per microliter（β = 0. 568, P 〈 0. 01 ） and quantities of total CD34^＋ cells （β = 0. 462, P 〈 0.01 ） in the G-BM. Conclusion The monocyte counts prior to the collection of G-BM may be a simple and practical indicator for harvests of CD34^＋ cells.

关 键 词：人重组粒细胞集落刺激因子 造血干细胞移植 CD34^+细胞 单核细胞 

分 类 号：R5[医药卫生—内科学]