HPLC-ELSD法测定贞芪扶正片黄芪甲苷的含量  

Determination of Astragaloside IV in Zhenqi fuzheng tablets by HPLC-ELSD

作　　者：吴杏梅[1] 廖华卫[2] 蔡先东[1] 陈树新[1] 陈翠环[1] 

机构地区：[1]广东省佛山市第一人民医院,528000 [2]广东药学院中药学院

出　　处：《中国现代药物应用》2008年第10期1-2,共2页Chinese Journal of Modern Drug Application

摘　　要：目的建立测定贞芪扶正片中黄芪甲苷含量的高效液相色谱-蒸发光散射检测器法（HPLC-ELSD）。方法采用Kromasil C18（250mm×4.6mm,5μm）的色谱柱;以甲醇-水（80∶20）为流动相;流速：1ml/min;ELSD检测条件：检测器漂移管温度为80℃,氮气压力为50psi,柱温为30℃。结果黄芪甲苷在1.5225-15.225μg范围内与峰面积分别取自然对数后呈良好的线性关系（r=0.9994,n=6）,平均回收率是99.98%（RSD=0.3%）。结论HPLC-ELSD法测定贞芪扶正片中黄芪甲苷含量简便、准确、可靠。Objective To determine astragaloside Ⅳ in zhenqi fuzheng tablet by HPLC-ELSD. Methods HPLC-ELSD was used in the quantitative analysis of astragaloside Ⅳ in the sample. The separationwas performed on Kromasil C18 （250 mm × 4.6 mm, 5 μm） column with a mixture of methanol-water（ 80： 20） as mobile phase and the temperature of column was 30℃. The flow rate was 1.0 ml/min. The temperature of drift tube for measurement was 80℃, and the flowrate of N2 was 50psi. Results The standard curve was linear in the range of 1. 522 5-15.22 5 μg for astragaloside Ⅳ （r =0. 999 4,n =6）. The average recovery was 99. 98% and RSD was 0. 3 %. Conclusion The method is simple, reliable, and is a good method to determinate astragaloside Ⅳ.

关 键 词：高效液相色谱-蒸发光散射检测器法 贞芪扶正片 黄芪甲苷 

分 类 号：R286[医药卫生—中药学]