油藏调剖菌TP-1的培养条件及岩芯模拟驱油研究  

Studies on Cultivation and Core Simulated Test of Bacillus TP-1 for Microbial Profile Modification

作　　者：刘静[1] 王君[1] 张宏伟[2] 马挺[1] 李国强[1] 梁凤来[1] 刘如林[1] 

机构地区：[1]南开大学生命科学学院天津市微生物功能基因组学重点实验室分子微生物学与技术教育部重点实验室,天津300071 [2]天津出入境检验检疫局,天津300457

出　　处：《微生物学通报》2008年第4期491-495,共5页Microbiology China

基　　金：国家自然科学基金(No.50674058)

摘　　要：从油井产出水中分离得到一株兼性厌氧芽孢杆菌TP-1,该菌株可在55℃的油藏温度生长,并代谢产生粘性多糖和气体。经培养条件优化,发酵液多糖产量可达5.5g/L,产气量为22mL/L。低浓度的CaCl2、MgSO4和AlCl3对多糖的生成有促进作用。岩芯模拟试验表明,该菌的注入可使岩芯压力增加,提高石油采收率7.37%。TP-1是一株性能良好的油藏调剖菌。A facultative anaerobic bacterium. Bacillus TP-1 isolated from oil field wastewater could produce viscous polysaccharide and gas under oil reservoir condition at 55℃.The polysaccharide can produced 5.5 g/L and the output of gas was 22 mL/L. The viscous polysaccharide production was favorable in the lower concentration of CaCl2, MgSO4 and AlCl3. The results of core simulated tests showed that pressure was increased and oil recovery was enhanced by 7.37% after the bacteria injection, TP-1 strain has most of the desirable properties for microbial profile modification.

关 键 词：芽孢杆菌 多糖 微生物调剖 岩芯模拟 

分 类 号：TE357.4[石油与天然气工程—油气田开发工程]