超高静压在琥珀酸生产菌株选育中的应用  

The Application of Ultra High Hydrostatic Pressure in the Screening of Succinic Acid Producing Strain

作　　者：姜岷[1] 陈可泉[1] 蔡婷[1] 吴昊[1] 韦萍[1] 

机构地区：[1]南京工业大学制药与生命科学学院材料化学工程国家重点实验室,南京210009

出　　处：《微生物学通报》2008年第4期561-564,共4页Microbiology China

基　　金：国家"863计划"项目(No.2006AA02Z235);国家自然科学基金项目(No.20606017);国家自然科学基金重点项目(No.20336010)

摘　　要：采用超高静压对一株产琥珀酸放线杆菌A3进行诱变育种,进一步提高其生产性能。考察了压力、变压速度、生长期对菌株致死率的影响。在压力为200MPa,50MPa/min变压速度的诱变条件下对稳定期菌株进行诱变,筛选到一株突变菌株产琥珀酸放线杆菌B19,琥珀酸产量达到32.2g/L,比出发菌株A3提高了17.9%,代谢副产物乙酸的产量降低了11.5%,经过6次传代表明突变菌株具有稳定的遗传特性。Actinobacillus succinogenes A3 was mutagenized by ultra high hydrostatic pressure for improving the production ability of succinic acid. The effects of pressure, rate of pressure changes and growth periods on the lethality rate of the strain were investigated. The strain in stationary phase was mutagenized at 200 MPa with the pressure changes at the rate of 50 MPa/min. As a result, a mutation strain Actinobacillus succinogenes B19 was obtained, and the concentration of succinic acid could reach 32.2 g/L, which was 17.9% higher than that of the original strain, and in the meanwhile the concentration of acetic acid was decreased by 11.5%. After six generation, the mutant also has good stability of descendiblity for succinic acid production.

关 键 词：超高静压 产琥珀酸放线杆菌B19 琥珀酸 诱变育种 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]