金黄葡萄球菌中毒休克综合征毒素1的研究  

Investigation on toxic shock syndrome toxin 1 from Staphylococcus aureus

作　　者：王敏[1] 李先平[1] 付炅[2] 范婧[2] 唐爱国[1] 

机构地区：[1]中南大学湘雅二医院检验科,湖南长沙410011 [2]中南大学湘雅医学院医学检验系,湖南长沙410011

出　　处：《中华微生物学和免疫学杂志》2008年第4期334-337,共4页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：湖南省自然科学基金（06JJ4051）

摘　　要：目的应用PCR技术，检测金黄葡萄球菌的mecA基因和染色体上编码中毒休克综合征毒素1（TSST-1）的tst基因，了解tst基因的携带情况。方法用PCR法对我院2006年8月-2007年5月临床分离的84株金黄葡萄球菌mecA基因和tst基因进行体外扩增，建立快速、特异、灵敏的检测产TSST-1耐甲氧西林金黄葡萄球菌（MRSA）的方法。结果成功的对金黄葡萄球菌mecA基因和埘基因进行了检测，并进行基因测序。在我院84株受检金黄葡萄球菌中，41株金黄葡萄球菌的mecA基因扩增呈阳性，阳性株占48．81％。16株tst基因阳性，阳性株占19．05％。10株金黄葡萄球菌同时扩增出mecA基因和tst基因，阳性株占24．39％（10／41）。结论tst基因阳性株在临床分离的耐甲氧西林金黄葡萄球菌中占有较高的比例，应予以足够重视。Objective To detect the mecA gene and tst gene of toxic shock syndrome toxin 1 （ TSST - 1 ） of Staphylococcus aureus by using PCR and to learn the cartier condition of tst gene. Methods The mecA gene and tst gene of Staphylococcus aureus strains that isolated from clinical sources in our hospital during August 2006 to May 2007 were amplified in vitro using PCR, and to establish the rapid, specific, and sensitive method of detecting tst gene of methicillin resistant Staphylococcus aureus （MRSA）. Results The mecA gene and tst gene were detected, and were made the gene sequencing successfully. Forty-one of 84 strains had mecA gene（48.81% ）, 16 of 84 strains had tst gene（ 19.05% ）, 10 of 84 strains had both of them, and the positive rate was 24.39% （ 10/41 ）. Conclusion The proportion of tst gene positive strains of MRSA is high in clinic, and it must be paid more attention.

关 键 词：金黄葡萄球菌 中毒体克综合征毒素1 MECA基因 tst基因 

分 类 号：R515[医药卫生—内科学]