三种类型CpG-ODN作为蛋白亚单位疫苗佐剂的比较研究  

Comparative study of the adjuvanticity of type-A, B and C CpG-ODN in mice

作　　者：张中洋[1] 王潇潇[1] 郝春生[1] 郑晓霞[1] 卜令楠[1] 许洪林[1] 

机构地区：[1]北京生物制品研究所病毒研究室,北京100024

出　　处：《中华微生物学和免疫学杂志》2008年第4期368-372,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的比较A、B和C3种类型CpG-ODN在动物模型中的疫苗佐剂活性，为设计高活性的新型人用疫苗佐剂提供理论基础。方法用不同CpG-ODN刺激体外培养的小鼠脾细胞，检测上清中的IFN-γ和IgM，从而确定3种类型的CpG-ODN。以基因工程乙肝表面抗原（HBsAg）为模型抗原、不同类型CpG-ODN为佐剂免疫BALB／c小鼠，用间接ELISA法检测被免动物血清中抗原特异性IgG、IgG1和IgG2a抗体。结果小鼠免疫结果表明，与A1（OH），对照组相比，3种类型CpG-ODN均具有良好的疫苗佐剂活性，但B和c型CpG-ODN诱生的抗原特异性IgG和IgG2a抗体水平远高于A型CpG-ODN。虽然A型CpG-ODN也能够显著增强总抗体水平，但并不改变IgG1和IgG2a抗体亚型的比值。这与B和c型CpG-ODN能够极大地促进TH1类免疫应答并降低IgG1／IgG2a比值明显不同，提示不同类型CpG-ODN可能通过不同机制起到疫苗佐剂作用。结论不同类型CpG-ODN具有不同疫苗佐剂活性，B和C型CpG-ODN在小鼠体内的体液免疫佐剂效果优于A型CpG-ODN。Objective To compare the adjuvanticity of type-A, B and C CpG-ODN in mice. Methods Three types of CpG-ODN were identified through in vitro stimulation of murine splenocytes with various CpG-ODN. BALB/c mice were immunized with HBsAg, together with different types of CpG-ODN, and antigen-specific IgG , IgG1 and IgG2a titers were measured 4 weeks later by indirect ELISA . Results Compared with the control group, all 3 types of CpG-ODN enhanced humoral immune response to HBsAg. However, type-B and C CpG-ODN induced much higher levels of antigen-specific IgG and IgG2a than type- A CpG-ODN. Type-C CpG-ODN induced a similar THl-biased immune response as type-B CpG-ODN, revealed by decreased IgG1 to IgG2a ratio. In contrast, although type-A CpG-ODN increased IgG titers, it did not switch the balance between TH1 and TH2 immune responses. Conclusion All 3 types of CpG-ODN can enhance the humoral immune response to vaccines, but their adjuvanticity could be mediated through different mechanisms.

关 键 词：CPG-ODN 乙肝表面抗原 佐剂 

分 类 号：R392[医药卫生—免疫学] R979.5[医药卫生—基础医学]