吻合血管的同种异体骨移植后移植物再血管化的研究  

An experimental study on revascularization after vascularized bone allograft

作　　者：陶圣祥[1] 陈振光[1] 谢昀[1] 郑晓晖[1] 杨玉华[1] 

机构地区：[1]武汉大学中南医院显微骨科 武汉大学医学部显微外科研究所,武汉市430071

出　　处：《中华显微外科杂志》2008年第2期119-121,I0004,共4页Chinese Journal of Microsurgery

摘　　要：目的探讨吻合血管的同种异体骨移植后移植物在宿主体内再血管化过程及其规律。方法建立吻合血管的同种异体骨移植修复兔股骨大段缺损的模型，实验分成两组，实验组（吻合旋股外血管股骨中上段等位异体骨移植组）和对照组（未吻合血管的股骨中上段等位异体骨移植组），对移植物骨膜、皮质骨及骨髓进行组织学切片，同时对微血管内皮细胞进行免疫组化染色，血管计数后，进行统计学分析，制备墨汁灌注标本，观察各部位微血管再生情况。结果实验组即可见骨膜、皮质骨和骨髓同时出现再血管化现象．而对照组则由浅至深逐渐出现再血管化现象．在术后2、4、8、16周实验组微血管密度分别为10．0±1．8、15．8±1．5、13．8±1．5、13．8±1．5，对照组分别为2．8±0．8、6．0±0．9、5．5±1．0、6．0±1．1，实验组明显高于对照组；墨染显示实验组墨染范围及程度明显强于对照组。结论吻合血管的异体骨移植能加快移植物再血管化的过程。Objectives To explore the process and regularity of graft after vascularized bone allograft. Methods An adult rabbit model of massive femoral defect was established and reconstructed with vascularized bone allograft based by laterial rotating femoral vessel. The experiments were carried out in two groups, the experimental group （vascularized bone allograft） and the control group （nonvascularized bone allograft）. Then observation on periosteum,cortex and marrow was performed after operation containing following items： （1）histological examination; （2）vessels counting and statistics analysis; （3）vessels regeneration in ink-infused specimen. Results The revascularization in the experimental group was observed synchronicly on periosteum, cortex and marrow after operation, while the phenomenon of vascularization took place from periosteum to marrow in the control group.The density of micro-vessel in experimental group were 10.0 ± 1.8,15.8 ± 1.5 and 13.8 ± 1.5,13.8 ± 1.5 respectivly after 2,4,8 and 16 weeks,and those were 2.8 ± 0.8,6.0± 0.9,5.5 ± 1.0,6.0 ± 1.1 in control group. The ink-infused experiment demonstrated a better revascularization in the experimental group. Conclusion The vascularization can promote revascularization of graft during bone allograft.

关 键 词：骨移植 再血管化 显微外科技术 兔 

分 类 号：R687.3[医药卫生—骨科学]