脱细胞牛心包种植人血管混合细胞构建组织工程心脏瓣膜  

Construction of tissue engineered heart valves based on decellularized bovine pericardium

作　　者：成少飞[1] 杨岷[1] 陈长志[1] 叶清[1] 薛松[1] 

机构地区：[1]上海交通大学医学院附属仁济医院心胸外科,200127

出　　处：《中华胸心血管外科杂志》2008年第2期108-111,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：本课题受教育部留学回国人员科研基金资助（03-406-01）

摘　　要：目的探讨在脱细胞牛心包上种植人血管混合细胞构建组织工程心脏瓣膜的方法与效果。方法将体外培养的人血管混合细胞种植在脱细胞牛心包材料上，观察种子细胞形态学和免疫组织化学变化和牛心包超微结构变化，并测定内皮细胞分泌t-PA和PAI-1的活性。结果种子细胞全层覆盖脱细胞牛心包表面，并浸润至组织内部生长；牛心包表面为扁平、片状、分层的细胞覆盖，细胞表面有少量绒毛和纤细的纤维结构。免疫组化显示内皮细胞Ⅷ因子、平滑肌细胞α-肌动蛋白、成纤细胞Fibronectin相关抗原呈阳性表达；种子细胞能分泌纤溶活性物质t-PA和PAI-1。结论人血管混合细胞种植后附着满意，与脱细胞牛心包生物相容性好，生长状况好，并具有内皮功能。Objective To evaluate the method and outcome of mixed human vascular cells seeding on decellularized bovine pericardium in constructing a tissue engineered heart valves （TEHVs）. Methods The mixed human vascular cells obtained by primary co-culture were seeded on decellularized bovine pericardium to construct a TEHVs. The morphologic and immunohistochemistry changes of the seeding cells, the ultramicrostructure of the decellularized bovine pericardium seeded with mixed human vascular cells were observed dynamically. Results The seeded cells overlaid on the decellularized bovine pericardium gradually, and with the time-lapse, the cells stratified and penetrated into the deep layer of the pericardium. The mixed human vascular cells were verified as endothelial cells （ECs）, smooth muscle cells （SMC） and fibroblasts by the immunohisto- chemistry. And the seeding cells were testified for their ability of secreting t-PA and PAI-1 by enzyme-labeled immunosorbent assay （ELISA）. The seeding cells on the pericardium were flat, lamellated or inordinate, and then stratified, with some floss and fibre. Conclusion The histocompatibility of the mixed human vascular cells and the decellularized bovine pericardium is satisfactory. The cells can grow well on the pericardium and possessed some functions of ECs.

关 键 词：组织工程 心脏瓣膜 脱细胞 牛心包 

分 类 号：R686[医药卫生—骨科学]