七氟醚对未成熟兔在体心肌缺血／再灌注血清IL-6、TNF—α的影响  

Effects of sevoflurane on serum IL-6 and TNF-α levels after regional myocardial ischemia-reperfusion in inmmture rabbits in vlvo

作　　者：黄瑾瑾[1] 张泽伟[2] 胡瑶琴[1] 胡智勇[1] 

机构地区：[1]浙江大学医学院附属儿童医院麻醉科,杭州310003 [2]浙江大学医学院附属儿童医院心胸外科,杭州310003

出　　处：《中华胸心血管外科杂志》2008年第2期112-114,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：本课题受浙江省教育厅科研项目（20061264）及浙江省科技厅科研项目（2004C23016）资助

摘　　要：目的研究七氟醚对未成熟兔在体心肌缺血／再灌注血清IL-6、TNF-α水平的影响。方法1—2周龄未成熟兔随机分为对照组（C组）、缺血／再灌注组（I／R组）和七氟醚预处理组（S组），每组8只。分别于缺血前（T0）、缺血30min再灌注前（L）、再灌注120min后（T2）采取静脉血，ELISA法检测血清中IL-6和TNF-α的水平；再灌注120min后取心肌组织，HE染色光镜下观察其病理学改变。结果T2时S组IL-6水平（66．07±24．12）pg／μl，低于I／R组的（200．37±93．07）pg/μl，P〈0．05；TNF-α水平（6．56±3．61）pg／μl，略低于I，R组（8．59±2．68）pg／μl，但差异无统计学意义（P〉0．05）。光镜下S组心肌组织病理改变较I/R组轻。结论七氟醚可通过降低血浆IL-6、TNF-α水平，减轻机体的炎症反应来对未成熟兔在体心肌缺血，再灌注损伤起保护作用。Objective To investigate the effects of sevoflttrane on serum cytokines TNF-α and KA5 levels in immature rabbits heart in vivo after regional myocardial isehemia-reperfusion （I/R）. Methods Twenty-four immature rabbits （one to two weeks old and weighting 290- 375 g） were randmly divided into three groups （8 animals each group） ： control group （C）; isehemia-repeffusion group （I/R） and evoflurane and isehemia-reperfusion group （S）. The animals were anesthetized with intramuscular injection of ketamine （80 mg/kg）. Chest was opened and heart was exposed. Left coronary artery was terapomrily occluded lmm inferior to left auricle for 30 min foUowed by 120 min reperfusion. Myocardial ischemia was confirmed by decoloration of apex. In group C, heart was exposed but left coronary artery was not occluded. In group I/R, left coronary artery was occluded for 30 rain followed by 120 min reperfusion. In group S, sevoflurane was inbreathed during operation, ischemia and reperfusion. The animals were sacrificed at the end of 120 min reperfusion. Blood samples were taken at the beginning of ischemia （TO）, 30 min of ischemia （T1） and 120 rnin of reperfusion for determination of sennn IL-6 and TNF-α levels （enzyme-linked immunosorbent assay ELISA）. Myocardium was obtained for histological examination, Results The serum IL-6 level in group S was lower than those in group I/R [ （66.07 ±24.12） pg/μl, （200.37±93.07） pg/μl]（P〈0.05）, but TNF-α level was same [（6.56±3.61） pg/μl, （8.59±2.68） pg/μl]（P〉0.05）. Microscopic examination showed that there were less destructive changes of myocardium in group S than those in group I/R.Conclusion Sevoflurane can attenuate the increase of serum IL-6 level during myoeardial ischemia-reperfusion in immature rabbit in vivo, so it may have protection on immature myocardial ischemia-reperfusion.

关 键 词：心肌灌注损伤 七氟醚 兔 心肌缺血 血清 

分 类 号：R686[医药卫生—骨科学]