HPLC法测定益心酮片中牡荆素鼠李糖苷的含量  

Determination of Vitexia-rhamnoside in Yixintong Tablet by HPLC

作　　者：张蓉[1] 陈有根[1] 

机构地区：[1]北京市药品检验所,北京100035

出　　处：《中国药品标准》2008年第2期150-152,共3页Drug Standards of China

摘　　要：目的:建立益心酮片中牡荆素鼠李糖苷的高效液相色谱含量测定方法;方法:采用岛津VP-ODS(4.6mm×250mm,5μm)色谱柱,流动相为四氢呋喃-异丙醇-1%甲酸溶液(12:4:84),检测波长为340nm;结果:牡荆素鼠李糖苷进样量在1.18～11.80μg之间线性良好,r=0.9998,加样回收率为98.58%,RSD为1.58%;结论:该方法重现性良好,结果准确可靠,可作为评价中成药益心酮片的重要参考方法。Objective： To develop an HPLC asssay for content determination of vitexia-rhamnoside in Chinese patent medicine Yixintong Tablets. Method： The separation was performed on a Shimadzu VP-ODS column （4. 6 mm × 150 mm, 5 μm）, and the mobile phase consisted of tetrahydrofunan, 2-pro- patiol and 1% formic acid （12： 4： 84） at the flow rate of 1.0 mL · min^- 1. The detection was carried out at 340 nm Results： The assay was validated in the weight range of 1.18 - 11.80 μg （r =0. 999 8）. The average recovery was 98.58% （RSD =1.58% ）. Conclusion： The method is accurate and reproducible, and can be used for determination of vitexia-rhamnoside in Yixinteng Tablet.

关 键 词：益心酮片 牡荆素鼠李糖苷 高效液相色谱法 

分 类 号：R963[医药卫生—微生物与生化药学] R259.41[医药卫生—药理学]