反相离子对高效液相色谱法测定硝酸益康唑溶液中硝酸益康唑的含量  

Determination of Ecoazole Nitrate in Econazole Nitrate Solution by Ion Pair RP-HPLC

作　　者：胡翮 郭丙炎 李昂 朱芹 

机构地区：[1]湘潭市药品检验所,湖南湘潭411100

出　　处：《中国药品标准》2008年第2期154-156,共3页Drug Standards of China

摘　　要：目的:建立反相离子对高效液相色谱法测定硝酸益康唑溶液的含量。方法:HPLC法,色谱柱为Diamonsil C18(4.6mm×200mm,5μm);流动相:含0.003mol.L-1庚烷磺酸钠的0.05mol.L-1的磷酸二氢钾溶液:甲醇(3:7)(磷酸调pH值至3.5);流速为1.0mL.min-1,检测波长:271nm;柱温:室温。结果:硝酸益康唑在浓度为0.20～0.55g.L-1时成线性关系,y=3.804×l04+1.474×106,r=0.9999平均回收率为101.18%,RSD为0.7%。结论:该法简便,可靠,准确,可作为硝酸益康唑溶液的含量测定方法。Objective：To establish an ion pair RP-HPLC method for determination of Econazole Nitrate. Method：The HPLC method was carried out with a D iamonsil C18 （4. 6 mm× 200 mm,5 μm）column and mobile phase consisted of 0. 05 mol · L^-1 potassium dihydro- gen phosphate solution containing 0. 003 mol · L^-1 sodium heptanesulfonate-methanol（3：7 ）, adjusted to pH 3.5 by phosphoric acid. The flow rate was 1.0mg · ml^-1 ,and the wavelength was set at 271 nm. Results：Econazole Nitrate standard curves were hner in the range of 0.20 - 0. 55 g · L^- 1 （ r = 0. 999 9）. The average recovery was 101.18% （ RSD = 0. 78% ）. Conclusion： The method is simple,reliable and accurate for content determination of Econazole Nitrate solution.

关 键 词：硝酸益康唑 反相离子对高效液相色谱法 含量测定 

分 类 号：R978.19[医药卫生—药品] R927.2[医药卫生—药学]