Cis-regulatory element-based genome-wide identification of DREB1/CBF targets in Arabidopsis  

作　　者：Huan Zhao Weizhong Liu Yong Hu Xianglin Liu Yikun He 

机构地区：[1]College of Life Science, Capital Normal University, Beijing 100037, China [2]College of Life Sciences, Shanxi Normal University, Linfen 041004, China

出　　处：《Progress in Natural Science:Materials International》2008年第5期579-583,共5页自然科学进展·国际材料（英文版）

基　　金：This work was supported by the National High-Tech Research Development Program (No.2O02AA2Z1001）; the National Natural Science Foundation of China （Grant No．30328003); PHR (IHLB)

摘　　要：Microarray analysis is used to identify transcriptional targets. However, the direct targets of transcription factors cannot be distinguished from indirect ones; further, genes with low-expression levels cannot be identified by this method. In the present study, we exploit the cis-element dehydration-responsive element （DRE） that is known to be responsible for the transcription of DRE binding factor 1 （DREB1） targets in the promoter region of all Arabidopsis genes. Putative targets whose promoters contain the elements were verified by both microarray and reverse transcription-polymerase chain reaction （RT-PCR） analysis. Five new DREB1/CBF direct targets were identified. Compared with traditional microarray analysis, our method is convenient and cost-effective for identifying the downstream targets of transcription factors.Microarray analysis is used to identify transcriptional targets. However, the direct targets of transcription factors cannot be distin- guished from indirect ones; further, genes with low-expression levels cannot be identi ed by this method. In the present study, we exploit the cis-element dehydration-responsive element (DRE) that is known to be responsible for the transcription of DRE binding factor 1 (DREB1) targets in the promoter region of all Arabidopsis genes. Putative targets whose promoters contain the elements were veri ed by both microarray and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Five new DREB1/CBF direct targets were identi ed. Compared with traditional microarray analysis, our method is convenient and cost-e ective for identifying the downstream targets of transcription factors.

关 键 词：ARABIDOPSIS CIS-ELEMENT Transcription factor DREB1/CBF Stress 

分 类 号：Q78[生物学—分子生物学]