ApoE基因敲除小鼠和C57BL/6J小鼠血脂及主动脉组织病理学的对比观察  

Observation on Blood-lipoid and Histopathological Changes of Aortic Root in Apolipoprotein-EKnockout Mice and C57BL/6J Mice Fed with Feed Rich in Fat

作　　者：程军[1] 李金平[1] 田卓[1] 胡厚源[1] 

机构地区：[1]第三军医大学西南医院心内科,重庆400038

出　　处：《实验动物科学》2008年第2期4-6,共3页Laboratory Animal Science

基　　金：国家自然科学基金资助课题(No.30770851)

摘　　要：目的高脂饲养的雄性载脂蛋白E基因缺陷小鼠动脉粥样硬化模型的建立。方法选取8周龄雄性ApoE-/-小鼠,以C57BL/6J小鼠为对照组,高脂饲养8周后测定血清中总胆固醇、甘油三酯、高密度胆固醇脂蛋白、低密度胆固醇脂蛋白含量;小鼠心脏主动脉根部石蜡切片,常规HE染色,观察和测量动脉粥样硬化斑块的组织学特点及病变面积。结果ApoE-/-小鼠血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇的含量显著高于同年龄同性别C57BL/6J小鼠,高密度脂蛋白的含量显著低于同龄同性别C57BL/6J小鼠;高脂饲养下16周龄ApoE-/-小鼠主动脉根部切面可见明显的脂质斑块。结论在该浓度的高脂饲料饲养条件下,ApoE-/-小鼠在16周龄已形成脂质斑块,是研究动脉粥样硬化等心血管疾病较佳的动物模型。Objective To develop atherosclerotic model of apolipoprotein-E （ApoE） knockout mice. Methods Six male 8-week-old ApoE-knockout mice were fed with atherogenic diet for 8 weeks, and 6 male 8-week-old C57BL/6J mice were used as controls. The mice were sacrificed, and blood was drawn for determination of serum levels of TG, TC, HDL-C, and LDL-C and their hearts and aortic root were subjected to paraffin sections stained with hematoxylin and eosine to examine atherosclerotic lesions in aortic root. Results Serum TG, TC, and LDL-C levels in ApoE- knockout mice were significantly higher than those in C57BL/6J mice, whereas the reverse was true for serum HDL-C level. Lipid plaques of atherosclerosis were seen in the sections of aortic root of the ApoE-knockout mice. Conclusion Atherosclerotic plaques have already generated in 16-week-old ApoE-knockout mice, which may be ideal model for research on cardiovascular diseases.

关 键 词：载脂蛋白E基因缺陷小鼠 动脉粥样硬化 血脂 

分 类 号：Q954-33[生物学—动物学]