纳米磷酸钙复合骨支架体内降解的实验研究  

In-vivo Degradation of Calcium Phosphate/Bioglass Nano-Composite Bone Tissue Engineering Scaffold

作　　者：许国华[1,2] 蔡舒[1,2] 于显著[1,2] 袁文[1,2] 叶晓健[1,2] 吕宏[1,2] 贾连顺[1,2] 李家顺[1,2] 

机构地区：[1]上海长征医院骨科,上海200003 [2]天津大学先进陶瓷与加工技术教育部重点实验室,天津300072

出　　处：《稀有金属材料与工程》2008年第A01期611-614,共4页Rare Metal Materials and Engineering

基　　金：国家自然科学基金(30400451);上海市纳米专项基金(05nm05014)资助

摘　　要：研究纳米磷酸钙复合氧化铝氧化镁生物玻璃骨组织工程支架在动物体内的降解特性,为进一步构建组织工程化纳米人工骨提供研究依据;本研究将合成灭菌后的纳米磷酸钙复合骨支架植入新西兰大白兔股部肌袋,并在植入4、8、12、16 w取材进行大体、组织学、电镜观察降解情况,16 w时将取出的材料煅烧,测量残余无机物含量,同时进行X线衍射实验,测量残余材料的晶相组成。结果是:合成的纳米磷酸钙复合骨支架孔隙率为80%,孔径200～450μm,抗压强度为3.8 MPa。材料在动物体内前8 w内降解较慢,生物力学强度减低不明显,12 w后降解明显加速,强度明显减低,并有羟基磷灰石成分出现,煅烧后残余无机物最少,16 w明显新骨形成,降解残余材料分布于新形成的骨组织内部,羟基磷灰石成分明显增多。结论显示纳米磷酸钙复合骨支架具有较好的降解性、生物相容性和诱导成骨作用,可作为骨组织工程的支架材料。Nano-powders of calcium phosphate and bioglass were synthesized by mechanical-chemical and precipitation method, and they were used to prepare calcium phosphate/bioglass nano-composite bone tissue engineering scaffold by organic template dipping method. Sterilized scaffolds were intramuscularly implanted into the thighs of New Zealand rabbits, and they were taken out from the killed animals at the 4th, 8th, 12th and 16th week after implantation to make general, histological and scanning electron microscope （SEM） observations on material degradation situation. All materials in muscular pouches were calcined at high temperature to gasify organic components, and the content of residual inorganic components was measured to determine material degradation. At the 4th week after implantation, fibrous tissue filled among material pores was observed with optical microscope and electron microscope, and there was no obvious change in pores and pore walls; at the 8th week, material pores were basically complete, but pore walls were broken; at the 12th week, collapsed wreck-like material pores were observed with electron microscope; at the 16th week, optical microscope observation revealed that residual degraded material was reconstructed and distributed in newly formed bone tissue, while electron microscope observation revealed that the bone scaffold was obviously reconstructed to form new pores with thin wall. Calcination result revealed no obvious weight changed at the 4th week, slight weight decreased at the 8th week, obvious weight decreased at the 12th week, and certain weight increased at the 16th week in comparison within the 12th week. Calcium phosphate/bioglass nano-composite material has good degradability, bio-compatibility and osteogenesis inducing effect, and can be used for bone tissue engineering scaffold.

关 键 词：纳米 磷酸钙 生物玻璃 组织工程 降解 

分 类 号：R318.17[医药卫生—生物医学工程]