高效液相色谱法测定人体血浆及尿液中多西环素的药物浓度  

HPLC determination of doxycycline in human plasma and urine

作　　者：李佩琼[1] 王九辉[1] 王小蒙[1] 

机构地区：[1]海南医学院药理教研室,海口571101

出　　处：《药物分析杂志》2008年第4期524-527,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建市人体血浆及尿液中多西环素药物浓度的高效液相色谱分析方法。方法:美他环素作为内标,待测血浆和尿液分别以高氯酸和甲醇沉淀蛋白,离心后取上清液进行高效液相色谱紫外检测,色谱柱为 Kromasil C_(18)柱(5μm,4.6mm×250mm);流动相为乙腈-水(30:70,含5 mmol·L^(-1)的柠檬酸),流速1.0 mL·min^(-1),检测波长350 nm。结果:本测定方法血浆和尿液的线性范围分别为O.1～6.13 mg·L^(-1)和0.085～13.62 mg·L^(-1),相关系数分别为0.9995和0.9991,血浆及尿液的相对回收率分别为95.2%～101.4%,95.6%～105.5%;日内日间 RSD 均小于10%,最低检测限分别为0.026 mg·L^(-1)及0.034mg·L^(-1)。结论:高效液相色谱法测定血浆与尿液中药物浓度简单、快速、可靠,可用于多西环素的药代动力学及生物利用度研究。Objective：To establish an HPLC method for the determination of doxycycline in human plasma and urine. Methods：The internal standard was methacycline. Plasma and urine sample was deproteinated with 18% per-chloric acid and methanol, respectively, then determined with HPLC and ultraviolet detector. The chromatographic separation was carried out on Kromasil-C18 column（5 μm ,4.6 mm×250 mm） with the mobile phase consisting of acetonitrile：water （30： 70,v/v） which containing 5 mmol·L^-1 citric acid,flow rate at 1.0 mL·min^-1. Detected wavelength was set at 350 nm. Results：The linear ranges of plasma and urine were 0.1-6.13 mg L^-1 and 0. 085-13.62 mg L^-1 ,respectively. The relative recoveries of doxycycline in plasma and urine were 95.2%- 101.4% and 95.6%-105.5% ,respectively. The intre-day RSD and the inter-day RSD were all less than 10%. Conclusion：The HPLC method is simple, rapid and accurate. It can be applied to pharmacokinetics and bioavailability research of doxycycline.

关 键 词：多西环素 高效液相色谱法 血浆 尿液 

分 类 号：R917[医药卫生—药物分析学]