HPLC法同时测定双黄消炎片中黄芩苷、药根碱、巴马汀及小檗碱的含量  

HPLC determination simultaneous of baicalin,jatrorrhizine,palmatine and berberine in Shuanghuang Xiaoyan tablets

作　　者：饶毅[1,2] 魏惠珍[1,2] 方海红[2] 梁佳[2] 杨世林[1] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006 [2]江西中医学院,南昌330006

出　　处：《药物分析杂志》2008年第4期537-539,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家十一五科技支撑计划资助项目(编号:2006BA108803-05)

摘　　要：目的:用 HPLC 法同时测定双黄消炎片中黄芩苷和3种生物碱类成分药根碱、巴马汀及小檗碱的含量。方法:色谱柱:Diamonsil^(TM)(钻石)C_(18)柱(250 mm×4.6 mm,5μm);流动相:0.1%磷酸(加三乙胺调 pH 至2.97)-乙腈(75:25);流速:1.0 mL·min^(-1);检测波长:345 nm。结果:黄芩苷、药根碱、巴马汀和小檗碱分别在0.13～2.7μg(r=0.9998),0.0097～0.19μg(r=0.9998),0.010～0.20μg(r=0.9999),0.020～0.40μg(r=0.9998)范围内线性关系良好;4个成分的平均回收率(n=9)分别为100.4%,99.7%,100.1%,100.4%。结论:本方法快速、简便,重复性好,可作为该制剂的质量控制方法。Objective：To determine the contents of baicalin,jatrorrhizine,palmatine and berberine in Shuanghuang Xiaoyan tablets by HPLC. Methods： Using an HPLC method with DiamonsilTM C18 column （250 mm × 4.6 mm,5μm）,0.1% phosphoric acid （triethylamine adjusted to pH 2.97 ） -acetonitrile （75：25） as mobile phase, flow rate was 1.0 mL·min^-1,with the detection wavelength at 345 nm. Results：The calibration curves of baicalin,jatrorrhizine, palmatine and berberine were linear within the range of 0. 13-2.7 μg（r=0.9998） ,0.0097-0.19 μg （r=0.9998） ,0. 010-0. 20 μg（r=0.9999） ,0. 020-0.40 μg（r=0.9998） respectively. The average recoveries （n =9） were 100. 4% ,99.7,100.1%, 100.4%. Conclusion：The method is rapid,sensitive and better reproducibility. It can be used to control the quality of Shuanghuang Xiaoyan tablets.

关 键 词：双黄消炎片 黄芩苷 药根碱 巴马汀 小檗碱 HPLC 

分 类 号：R917[医药卫生—药物分析学]