HPLC法测定板蓝根药材中靛蓝和靛玉红的含量  

HPLC determination the contents of indigo and indirubin in Radix Isatidis

作　　者：范丽芳[1] 张兰桐[1] 袁志芳[1] 许慧君[1] 何伟[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,石家庄050017

出　　处：《药物分析杂志》2008年第4期540-543,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：河北省自然科学基金项目(303453)

摘　　要：目的:采用 HPLC—UV 法测定板蓝根中靛蓝和靛玉红含量。方法:将板蓝根经氯仿回流提取,然后用 HPLC 测定。色谱柱为 Diamonsil^(TM)C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸梯度洗脱,流速为1.0 mL·min^(-1),检测波长为300 nm,柱温为30℃。结果:靛蓝线性范围为0.1488～9.520μg·mL^(-1)(r=0.9999,n=7),平均回收率为97.8%,RSD=1.7%(n=6);靛玉红线性范围为0.2539～16.25μg·mL^(-1)(r=0.9995,n=7),平均回收率为98.5%,RSD=1.64%(n=6)。结论:所用方法准确可靠,适合于板蓝根药材的质量控制。Objective：To develop a method for the determination of indigo and indirubin in Radix Isatidis. Method：Radix Isatidis was extracted with trichlormethane and the chromatographic procedure was carried out with Dia-monsil^TM C18 （250 mm×4.6 mm,5μm）. The analytical column with gradient elution was a mixture consisting of acetonitrile and 0.05% phosphoric acid as mobile phase. The flow rate was 1.0 mL·min^-1. The detection wave-length was set at 300 nm. The temperature of column was 30℃. Results： The linear range of the indigo was 0.1488-9.520 μg·mL^-1（r=0.9999,n=7） ,and the average recovery was 97.8% ,RSD was 1.7% （n=6）. The linear range of the indirubin was 0. 2539-16.25 μg·mL^-1（r=0.9995, n=7）,and the average recovery was 98.5%, RSD was 1.6% （n=6）. Conclusion： This method of the operation is accurate, reliable and can be used to control the quality of Radix Isatidis＇ crude drug.

关 键 词：板蓝根 靛蓝 靛玉红 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]