TLC法测定贯叶连翘粗提物中金丝桃素的含量  

TLC determination of hyperforin in crude extract of Hypericum perforatum L.

作　　者：杨李[1] 张生万[2,1] 杜文[1] 王伟[1] 李美萍[2] 

机构地区：[1]山西大学化学化工学院,太原030006 [2]山西大学生命科学与技术学院,太原030006

出　　处：《药物分析杂志》2008年第4期608-610,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：山西省科委攻关项目(2006031204);山西省中青年拔尖人才基金(04)

摘　　要：目的:建立薄层色谱扫描(TLC)测定贯叶连翘粗提物中金丝桃素含量的方法。方法:在 Na_2CO_3溶液处理过的硅胶 G板上用石油醚-乙酸乙酯-甲醇(1:4:2)混合展开剂上行展开后,采用日本岛津 CS-930型薄层色谱扫描仪,在检测波长591nm 处进行 TLC 定量测定。结果:金丝桃素点样量在0.0～14.4μg的范围内与峰面积呈良好的线性关系,r 为0.9892,回收率范围为96.4%～1130.1%。结论:本方法操作简便、快速,结果准确可靠。Objective：Building a method to determine hyperforcin in Hypericam perforatum L. with TLC Scanning. Methods：The sample was separated on silica （G） with mixtime solvent of petroleum ether/EtOAc/CH3 OH（1：4：2） , the silica G is treated by the Na2CO3 solvent. The CS-930 scanning densitometer of Shimadzu was selected. The detection wavelength was 591 nm. Result：The calibration curves were linear in the range of 0.0-14.4 μg for hyperforcin（r=0.9892）. The range of recoveries were 96.4%-100.1%. Conclusion：The method is rapid,accurate and reliable.

关 键 词：薄层色谱扫描 贯叶连翘粗提物 金丝桃素 

分 类 号：R917[医药卫生—药物分析学]