细胞复合β-磷酸三钙生物陶瓷修复软骨缺损的实验研究  

Experiment study of the repairing of cartilage defects using cells with bioceramic scaffold

作　　者：张磊[1] 孙水[1] 任强[1] 丁远景[1] 

机构地区：[1]山东省立医院

出　　处：《中国矫形外科杂志》2008年第9期698-701,共4页Orthopedic Journal of China

摘　　要：[目的]通过将骨髓间充质干细胞(MCSc)诱导的具有软骨细胞、成骨细胞表型的细胞接种到三维多孔β-磷酸三钙(β-TCP)生物陶瓷支架材料上,体外构建骨软骨复合体,探讨以β-TCP为载体建造组织工程化软骨修复骨软骨缺损的可行性。[方法]将β-TCP多孔陶瓷加工成圆柱状,并将其作为构建人工软骨的细胞支架。在支架材料上分别接种从犬骨髓干细胞培养成的具有软骨细胞、成骨细胞表型的细胞,将细胞-支架复合体共同培养1周后,移植到犬关节软骨缺损处。植入后12、16周末取材,进行大体观察、组织学及组织化学等观察。[结果]复合体体内移植后,在犬关节软骨缺损处有新生软骨形成,形成的软骨基本保持了支架材料原有形态。[结论]β-TCP多孔陶瓷可作为支架材料,复合细胞后具有修复软骨缺损的作用。[ Objective] To evaluate the feasibility of repairing cartilage defect with tissue -engineered cartilage , which was made of chondrocytes and osteoblast seeded onto a biodegradable porous bioceramic β- tricalcium phosphate （β- TCP） . [ Method ] A porous bioceramic template of β-TCP was created in the shape of a circular cylinder. Chondrocytes and osteoblasts induced from dog bone marrow stem cells were seeded onto the β-TCP and then kept them growing together for 1 week prior to transplantation into the cartilage defects. After 12 , 16 weeks , the specimens were harvested and examined macroscopically, histologically and immunohistochemically. [ Result] Gross morphological and histological analysis of the specimens from the β-TCP complex demonstrated new cartilage construction. The result was better than that in control group. [ Conclusion ] These findings suggest that porous bioceramic （ β-TCP） is a good ＂matrix＂ for chondrocytes and osteoblasts, and can be used for cartilage engineering.

关 键 词：组织工程 生物陶瓷 软骨修复 软骨细胞 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程] R687.3[医药卫生—基础医学]