新生小鼠卵巢移植雄鼠肾囊下卵泡的生长发育  

Follicular growth and development of the neonatal mouse ovaries grafted in the male kidney capsule

作　　者：袁安文[1] 彭南妮[1] 王乃东[1] 许道军[1] 薛立群[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《动物学报》2008年第2期265-270,共6页ACTA ZOOLOGICA SINICA

基　　金：湖南省重点科技项目(No.01NKY1002-01);湖南农业大学科研基金项目(No.SK0344)资助~~

摘　　要：将1日龄小鼠卵巢移植入成年雄鼠肾囊下,分别于移植后18d、36d回收移植卵巢进行形态学、组织学观察,以评价卵巢移植体在成年雄性受体小鼠体内生长及卵泡发育潜能。结果表明:移植体生长增大,有各级生长卵泡发育;18日龄移植体平均直径为1881.1μm±204.7μm,与1日龄卵巢相比差异极显著(P<0.01),卵泡发育到有腔卵泡阶段;36日龄移植体平均直径达2575.3μm±466.4μm,显著大于18日龄移植体(P<0.01),有成熟卵泡出现,未观察到黄体;从移植体分离到GV期卵母细胞和卵丘卵母细胞复合体。研究表明1日龄小鼠卵巢移植体在雄性受体生理环境中具有正常生长发育和形成成熟卵泡的潜能。To evaluate the follicular developmental competence of neonatal mouse ovaries grafted into male recipient mice, ovaries from 1-day-old mice were transplanted underneath the kidney capsule of male recipient mice and were collected 18 and 36 days later for observation of histology and morphology. The retrieved grafts were significantly larger （ P 〈 0.01 ） when compared with 1- day-old ovaries and contained many growing follicles. The average diameter of the 18-day and 36-day grafts was 1881.1μm ± 204.7 μm and 2575.3 μm ± 466.4 μm respectively （ P 〈 0.01 ）. The follicles in the 18-day and 36-day grafts developed to antral and mature follicles respectively, without formation of corpora lutea. Oocytes at the germinal vesicle stage were isolated from the grafted ovaries. In conclusion, this work has shown that the physiological environment of male mice can support the development of follicles in allografts of neonatal mouse ovaries

关 键 词：小鼠 移植 卵巢 卵泡 

分 类 号：S865.13[农业科学—野生动物驯养]