农杆菌介导的高效水稻遗传转化体系的研究  

A Highly Efficient Agrobacterium-mediated Rice Transformation Method

作　　者：郑杰[1] 

机构地区：[1]湖南农业大学细胞工程实验室,湖南长沙410128

出　　处：《湖南农业科学》2008年第2期6-7,10,共3页Hunan Agricultural Sciences

基　　金：国家863计划项目(2001AA225051)

摘　　要：以水稻基因组测序品种日本晴为研究材料,对根癌农杆菌介导的水稻转化系统进行了优化。结果表明,对水稻种子采用2.5%次氯酸钠灭菌30 min的处理,愈伤组织的诱导率最高;获得的胚性愈伤组织经过6-7 d的继代培养后再转化,转化效率最高;感染时,发杆菌浓度OD600值为0.145-0.190时,转化效率较高;将抗性愈伤组织进行7 d的暗培养将大大提高分化效率。对获得的大量转化体进行初步分析,GUS表达率达到30%。The rice transformation method mediated by agrobacterium tumefaciens was optimized using rice genome sequencing variety nipponbare as test material, The results showed that the frequency of callus induction was the highest when seeds sterilized in 2.5 % sodium hypochlorite solution for 30 minutes, The transformation efficiency was the highest when embryogenic caUi sub - cultured for 6 - 7 days. The optimal bacteria concentration of callus transformation was OD600 = 0,145 - 0. 190. With 7 days cultivation in dark, the differentiation efficiency of the resistant calli improved sharply. Under these optimal condittons, stable GUS expression on calli transformed was 30 % .

关 键 词：根癌农杆菌 水稻 遗传转化 

分 类 号：S188.165.11[农业科学—农业基础科学]