马铃薯品种“大西洋”高效再生体系的建立  

Establishment of Efficient Regeneration Systerm from Potato Variety "Atlantic"

作　　者：彭昕琴[1] 胡家金[1] 霍妙娟[1] 

机构地区：[1]湖南农业大学生物科学与技术学院,湖南长沙410128

出　　处：《湖南农业科学》2008年第2期26-28,共3页Hunan Agricultural Sciences

基　　金：湖南农业大学人才基金(2005WD25);湖南省教育厅资助项目(03C613)

摘　　要：以马铃薯品种"大西洋"为试材,进行了叶片和茎段的离体再生研究。结果表明,叶片与茎段愈伤组织最佳诱导培养基分别为MS+6BA2.5 mg/L+NAA0.3 mg/L和MS+6BA2.5 mg/L+NAA0.2 mg/L,诱导率均达100%。叶片与茎段愈伤诱导不定芽分化的最佳培养基分别MS+6 BA3.0 mg/L+NAA0.1 mg/L+GA35.0 mg/L和MS+6 BA2.0 mg/L+NAA0.05 mg/L+GA35.0 mg/L,不定芽分化率分别达100%和80%。诱导试管苗生根的最佳培养基为1/2 MS+0.2 mg/LNAA,生根率为100%,试管苗移栽成活率为91%。The regeneration system of potato variety Atlantic was studied with leaves and stems as explants. The results showed that the optimal medium for callus induction from leaf and stem were MS ＋ 6BA 2.5 mg/L ＋ NAA0.3 mg/L and MS ＋ 6BA 2.5 mg/L＋ NAA 0.2 mg/L respectively, the callus induction rates all reached to 100% .The optimal medium for adventitious bud differentiation from leaf- derived calluses and stem segment - derived calluses was MS ＋ 6BA3.0mg/L ＋ NAA 0.1 mg/L ＋ GA 35.0 mg/L and MS＋ 6BA 2.0 mg/L＋ NAA 0.05 mg/L＋ GA 35.0 mg/L respectively. The adventitious bud differentiation rate reached to 100% and 80% respectively. The optimal medium for rooting induction from tube plantiets was 1/2MS ＋ NAA 0.2 mg/L, and the rooting rate was 100% .The survival rate of tube plantlets after transplantation reached to 91%.

关 键 词：叶片 茎段 愈伤组织 植株再生 

分 类 号：S188.165.32[农业科学—农业基础科学]