刺参糖胺聚糖对HepG2.2.15细胞分泌HBsAg和HBeAg的影响  

The effect of the stichopus japomicus selenka Glycosaminoglycans on HBsAg and HBeAg in the HepG2.2.15 cells

作　　者：蒋泽伟[1] 辛永宁[2] 罗兵[1] 宣世英[2] 

机构地区：[1]青岛大学医学院,266071 [2]山东省青岛市市立医院,266011

出　　处：《实用医学杂志》2008年第8期1289-1291,共3页The Journal of Practical Medicine

基　　金：山东省科技攻关计划资助项目(编号:2006gg2205019)

摘　　要：目的:研究刺参糖胺聚糖对HepG2.2.15细胞HBsAg和HBeAg的抑制作用。方法:采用不同浓度的刺参糖胺聚糖作用于HepG2.2.15细胞,MTT法检测药物的细胞毒性,ELISA法检测培养上清中的HBsAg和HBeAg。结果:刺参糖胺聚糖对HepG2.2.15细胞分泌HBsAg和HBeAg的抑制作用存在时间和浓度依赖性。与对照组相比,给予8、4、2及1mg/mL4种不同浓度的刺参糖胺聚糖作用3、6d后,各药物处理组均能显著抑制HBsAg和HBeAg的分泌(P<0.01)。结论:刺参糖胺聚糖具有一定的抗HBV活性。Objective To study the effects of stichopus japomicus selenka Glycosaminoglycans （SJ-GAG） on hepatitis B virus （HBV） in vitro. Methods The human hepatoma（Hep）G2.2.15 cell line stably expressed hepatitis B virus particles in culture. The cells were exposed to different concentrations of SJ-GAG, respectively. Methyl, thiazolyl tetrazolium（MTF） assay was used to evaluate the cytotoxicity of the drugs. HBsAg and HBeAg were assessed by enzyme-linked immunosorbent assay （ELISA）. Results The exposure of HepG2.2.15 cells to SJ-GAG resulted in a time-and concentration-dependent inhibition of HBsAg and HBeAg secretion. After exposed to four different concentrations of SJ-GAG for 3 and 6 days, the inhibition rates of extracellular HBsAg and HBeAg of each concentration increased significantly（P〈0.01 ）. Conclusion SJ-GAG may be a new modality to treat hepatitis B infection.

关 键 词：肝炎病毒 乙型刺参糖胺聚糖 HEPG2.2.15细胞 药物处理 

分 类 号：R285[医药卫生—中药学]