雷公藤内酯醇诱导T淋巴细胞凋亡时Fas／FasL的表达  

Expression of Fas/FasL in triptolide-induced T lymphocyte apoptosis

作　　者：刘冬舟[1] 褚爱春[2] 齐晖 谭艳红[1] 肖学吕[1] 马鹏程[4] 

机构地区：[1]暨南大学第二临床医学院,广东省深圳市人民医院风湿免疫科,518020 [2]武汉大学人民医院,430060 [3]暨南大学第二临床医学院,广东省深圳市人民医院临床医学研究中心,518020 [4]中国医学科学院皮肤病研究所,南京市210042

出　　处：《实用医学杂志》2008年第8期1295-1297,共3页The Journal of Practical Medicine

基　　金：深圳市科技计划项目资助项目(编号:200405009,200702003)

摘　　要：目的:检测Fas/FasL在雷公藤内酯醇诱导的T淋巴细胞凋亡中的表达。方法:培养人T淋巴细胞,10、20、30μg/L的雷公藤内酯醇分别刺激细胞4、8、16h,流式细胞仪DNA分析、FITC-Annexin Ⅴ binding/PI染色检测细胞凋亡,Western blot分析检测Fas/FasL蛋白的表达。结果:雷公藤内酯醇以剂量和时间依赖的形式诱导T淋巴细胞凋亡,雷公藤内酯醇诱导T淋巴细胞凋亡的同时伴随有Fas和FasL表达的上调,同样,雷公藤内酯醇诱导Fas和FasL表达呈现剂量和时间依赖性。结论:雷公藤内酯醇以剂量和时间依赖的形式诱导人T淋巴细胞凋亡,雷公藤内酯醇诱导T淋巴细胞凋亡的信号通路可能是通过Fas/FasL途径激活的。Objective To evaluate the expression of Fas/FasL in triptolide-induced T lymphocyte apoptosis. Methods Jurkat cells were incubated in cell culture media containing 10, 20, 30 μg/L triptolide for 4, 8, or 16 h. Apoptosis was assessed by flow eytometrie DNA analysis and FITC-Annexin V binding/PI staining. The protein expression of Fas/FasL was determined by Western blot analysis. Results Triptolide induced apoptosis in cultured Jurkat cells in a dose-and duration-dependent manner. Fas and FasL production were upregulated in cultured Jurkat cells treated with triptolide in a dose-and duration-dependent manner. Conclusions Triptolide induces apoptosis in cultured Jurkat cells in a dose-and duration-dependent manner. Jurkat cells apoptosis induced by triptolide may be activated through Fas/FasL interaction.

关 键 词：雷公藤内酯 T淋巴细胞 细胞凋亡Fas/FasL 

分 类 号：R285[医药卫生—中药学]