华北半叶紫菜藻胆体的光谱特性和光能传递的研究  

STUDIES ON THE SPECTRAL PROPERTIES AND ENERGY TRANSFER OF PHYCOBILISOME FROM PORPHYRA KATADAI VAR. HEMIPHYLLA

作　　者：路荣昭[1] 刘斌[1] 王淑芝[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《海洋与湖沼》1990年第5期480-484,共5页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金

摘　　要：红藻的光合器是由叶绿体和藻胆体组成的。藻胆体由多种藻胆蛋白和在结构上起连接作用的无色蛋白或多肽所组成。藻胆体与类囊体片层紧密结合,在单位类囊体的片层上藻胆体的数目因生物种类不同及其所处环境条件的不同而异;一般为400—1200个/μm^(-2)。 藻胆体的功能是捕获光能和将光能传递给类囊体片层上叶绿素a,以进行光合作用。Absorption spectra, fluorescence emission spectra and excitation spectra of phycobilisome from Porphyra katadai var. hemiphylla were studied. The five bands in absorption spectrum of phycobilisome were located at 500, 550, 568, 620 and 650 nm The room temperature fluorescence emission maximum of intact phycobilisome was located at 672 nm; that of non-intact phycobilisome was at 578 nm. There were three bands in the liquid nitrogen temperature fluorescence emission spectrum of phycobilisome: F580, F632 and F684, characteristic of R-phycoerythrin, R-phycocyanin and allophycocyanin-B respectively. The relative fluorescence intensity of F684 of intact phycobilisome was very high, and those of F580 and F632 were low. The relative fluorescence intensity of F684 in non-intact phycobilisome was decreased, and those of F580 and F632 were increased.Our research results indicated that the energy migration from R-phycoerythrin and R-phycocyanin to allophycocyanin-B in dissociated phycobilisome was inefficient; and showed that the fluorescence emission spectra can be used to identify the intactness and dissociation of phycobilisome.The light energy transfer among phycobiliproteins in phycobilisome was discussed.

关 键 词：半叶紫菜 藻胆体 光谱 物性 光能 

分 类 号：Q949.292.1[生物学—植物学]