蝴蝶兰叶片组织培养中抗褐化的研究  

Research of Preventing Leaves Browning on the Tissue Culture of Phalaenopsis

作　　者：聂卫卓[1] 贝丽霞[1] 

机构地区：[1]黑龙江八一农垦大学,大庆163319

出　　处：《黑龙江八一农垦大学学报》2008年第1期28-31,共4页journal of heilongjiang bayi agricultural university

摘　　要：研究了不同基本培养基、吸附剂、抗氧化剂对蝴蝶兰叶片组培过程中褐化的影响,结果表明:在叶片诱导类圆球茎过程中,VW培养基是最佳基本培养基,有效的降低了褐化率,类圆球茎的诱导率最高;培养基中添加活性碳2g·L-1时,褐化率最低为34.5%;添加抗坏血酸300mg·L-1时,褐化率最低为40.6%。Different kinds of basic mediums, adsorbents and antioxidants which prevent vitro leaves from browning in tissue culture Phalaenopsis were studied. The results showed that： in the process of PLB （protocorm-like body） induced from leaves, VW medium was the best one that reduced effectively browning rate, and the inducing rate of PLB in VW mediums was the highest in the five ones. The browning rate as 34.5% was the lowest when the medium was added 2g/L activated charcoal and the browning rate as 40.6% was the lowest when was added 300mg/L vitamin C.

关 键 词：蝴蝶兰 叶片 组织培养 抗褐化 

分 类 号：S682.31[农业科学—观赏园艺]