3,5-二硝基水杨酸比色法测定红毛五加中多糖的含量  

Quantitative Detemination of AGP by DNS Method

作　　者：章丽华 张利平 胡锦群 冯丽娟 张龙清 张莅峡[3] 

机构地区：[1]海南亚洲制药有限公司药物研究所,浙江金华321017 [2]浙江省丽水市医院,浙江丽水323000 [3]中国中医科学院基础理论研究所,北京100700

出　　处：《中国中医药信息杂志》2008年第5期49-51,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立红毛五加中多糖的含量测定方法。方法采用3,5-二硝基水杨酸比色法(DNS法),测定红毛五加中的还原糖和总糖的含量,并计算出总多糖的含量。结果以葡萄糖为对照品,线性范围为46.6～233.0mg/L(r=0.9992),加样回收率为99.0%～102.1%;红毛五加中总多糖含量1.3～1.9mg/g。结论本方法灵敏、稳定、重复性好,有助于红毛五加质量控制方法的研究。Objective To establish a method for the quantitative determination of AGP （Acanthopanax giraldii Harms polysaccharides）. Methods AGP content was determined by DNS Method. The absorptions of processed samples were tested at the wavelength of 520 nm. Results Contrast to glucose, the linear range was 46.6-233.0 mg/L （r=0.999 2）, the average recovery was 99.0%-102.1%, RSD=3.52%, n =5. The polysaccharides measured in 3 batches of samples were 1.3- 1.9 mg/g. Conclusion The method is sensitive, steady with good repeatability, and help to the qulity control of Acanthopanax giraldii Harms.

关 键 词：红毛五加多糖 葡萄糖 3 5-二硝酸水杨酸比色法 

分 类 号：R284.1[医药卫生—中药学]