高效液相色谱法测定双黄连口服液中绿原酸、黄芩苷、连翘苷、汉黄芩苷的含量  

Determination of Chlorogenic Acid, Baicaliin, Forsythin and Wogonin in Shuanghuanglian Oral Liquid by HPLC

作　　者：黄敬群[1] 杨娟[1] 李红[1] 

机构地区：[1]中国人民解放军第252医院,河北保定071000

出　　处：《中国中医药信息杂志》2008年第5期53-55,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立同时测定双黄连口服液中绿原酸、黄芩苷、连翘苷和汉黄芩素含量的高效液相色谱法。方法采用VP-ODSC18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-0.2%磷酸溶液梯度洗脱;流速:1.0mL/min;检测波长:278nm。结果绿原酸在0.44～6.60μg范围内线性关系良好(r=0.9991),平均回收率为97.0%;黄芩苷在0.52～6.18μg范围内线性关系良好(r=0.9991),平均回收率为98.98%;连翘苷在0.20～2.04μg范围内线性关系良好(r=0.9993),平均回收率为103.55%;汉黄芩素在0.13～1.76μg范围内线性关系良好(r=0.9991),平均回收率为96.49%。结论本方法简便可行、准确、快速,可用于双黄连口服液中上述4种成分的含量测定。Objective To establish a HPLC method for the determination of chlorogenic acid, baicaliin, forsythin and wogonin in Shuanghuanglian oral liquid. Methods The column was VP-ODS C18 （250 mm× 4.6mm, 5μm）. The mobile phase consisted of acetonitrile-0.2% phosphoric acid with gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was at 278 nm. Results The calibration curves were linear within the range of 0.4446.60 μg （r =0.999 1） for chlorogenic acid, 0.5246.18 μg （r =0.999 1） for baicaliin, 0.2042.04 μg （r=0.999 3） for forsythin （r=0.999 3） and 0.1341.76 μg （r=0.999 1） for wogonin, respectively. The average recovery of them were 97.0% （RSD= 1.1%）, 98.98% （RSD= 1.1%）, 103.55% （RSD = 1.1%） and 96.49% （RSD = 1.1%）, respectively. Conclusion The method is simple, practicable, accurate and rapid. It can be applied to content determination of chlorogenic acid, baicaliin, forsythin and wogonin in Shuanghuanglian oral liquid.

关 键 词：双黄连口服液 绿原酸 黄芩苷 连翘苷 汉黄芩素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]