慢性乙型肝炎病毒负荷与外周血单个核细胞激活诱导细胞死亡及FasL表达的关系  

Serum HBV DNA level and activation-induced cell death of peripheral blood mononuclear cells and expression of FasL in chronic hepatitis B patients

作　　者：熊英[1] 王瑶芬[1] 

机构地区：[1]南昌大学第一附属医院感染科,江西南昌330006

出　　处：《临床荟萃》2008年第9期618-620,共3页Clinical Focus

基　　金：江西省卫生厅资助项目(0303006)

摘　　要：目的探讨慢性乙型肝炎不同HBV DNA负荷与外周血单个核细胞(PBMC)激活诱导细胞死亡(AICD)及FasL表达的关系。方法常规无菌分离30例慢性乙型肝炎患者和13例健康献血员PBMC,各分两组培养72小时,其中一组加入植物血凝素(PHA)培养后经碘化丙啶(PI)染色,另一组未加PHA经PE-抗FasL标记,流式细胞仪分别检测PBMC凋亡率及FasL表达阳性细胞的百分率。结果慢性乙型肝炎患者与健康对照组PBMC凋亡率及其FasL表达阳性细胞的百分率比较差异均有统计学意义,(26.06±7.77)%vs(14.66±3.27)%,(0.846±0.299)%vs(0.432±0.126)%(t值分别为4.93,4.65,P<0.01)。HBV DNA高水平组慢性乙型肝炎患者PBMC凋亡率及其FasL表达阳性细胞的百分率显著高于低水平组,分别为(33.73±5.44)%vs(25.61±5.05)%vs(18.85±4.05)%,(1.055±0.224)%vs(0.880±0.285)%vs(0.603±0.208)%(H值分别为23.29,8.92,P<0.05)。慢性乙型肝炎患者PBMC凋亡率与其FasL表达呈正相关(r值为0.70,P<0.05)。结论慢性乙型肝炎患者PBMC存在AICD现象,HBV感染可激发外周血单个核细胞FasL的表达;慢性乙型肝炎患者HBV DNA负荷与外周血单个核细胞AICD及FasL表达水平呈正相关。Objective To investigate the relationship of serum HBV DNA level with activation-induced cell death （AICD） and the expression of FasL in peripheral blood mononuclear cells （PBMCs） of chronic hepatitis B patients. Methods PBMCs were isolated from 30 patients with chronic hepatitis B and 13 healthy blood donors. The PBMCs were divided into two groups and were cultured for 72 hours in vitro. One group was stimulated with phytohemagglutin（PHA）, the cultured PBMCs were stained by PI and the apoptotic rate was assayed by flow cytometry. The other group without stimulation of PHA was marked by PE anti-human FasL and the expression of Fas in PBMCs was also assayed by flow cytometry. Results The apoptotic rate of the PBMCs and the expression of FasL in chronic hepatitis B were significantly higher than those of healthy blood donors＂ group （26.06± 7.77） % vs （14.66± 3.27） %, （0. 846±0. 299） % vs （0. 432±0. 126）% （ t = 4.93,4.65, P d0.01）. The apoptotic rate of the PBMCs and the expression of FasL were significantly higher in chronic hepatitis B patients with high serum HBV DNA level （33.73 ± 5.44） % vs （25.61 ±5.05）% vs （18.85 ±4. 05） 0%, （1. 055 ± 0. 224） % vs （0. 880± 0. 285） %vs （0. 603±0. 208） % （ H = 23.29,8.92, P 〈0.05）. The apoptotic rate of the PBMCs was correlated with the expression of FasL （ r = 0.70, P 〈0.05）. Conclusion AICD in PBMCs is found in chronic hepatitis B. The expression of FasL in PBMCs is activated by chronic hepatitis B virus infection. The serum HBV DNA level is correlated with AICD and the expression of FasL of the PBMCs in chronic hepatitis B patients.

关 键 词：肝炎 乙型 慢性 细胞凋亡 免疫法 DNA 

分 类 号：R512.62[医药卫生—内科学]